Topical Application of Activity-based Probes for Visualization of Brain Tumor Tissue
Topical Application of Activity-based Probes for Visualization of Brain Tumor Tissue
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DOI：
10.1371/journal.pone.0033060
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发表时间：
2012-03-13
期刊：
影响因子：
3.7
通讯作者：
Basilion, James P.
中科院分区：
文献类型：
--
作者：
Cutter, Jennifer L.;Cohen, Nathan T.;Basilion, James P.
几位研究人员已经展示了系统输送光学成像探针在小动物癌症模型中成像肿瘤的实用性。在这里，我们展示了一种在手术中成像肿瘤和肿瘤边缘的创新方法。具体来说，我们表明局部应用于肿瘤和周围正常组织的光学成像探针可以快速区分组织之间的差异。与系统输送光学成像探针（随时间均匀地标记肿瘤）相比，局部探针应用可在应用后5分钟内检测到快速而强大的探针激活。重要的是，标记主要与肿瘤周围空间相关。该方法为肿瘤和潜在浸润性肿瘤细胞的快速可视化提供了一种手段，并在肿瘤组织的定向手术切除中具有潜在的应用前景。此外，该技术可用于手术切除任何具有半胱氨酸组织蛋白酶活性差异调节的肿瘤。
Several investigators have shown the utility of systemically delivered optical imaging probes to image tumors in small animal models of cancer. Here we demonstrate an innovative method for imaging tumors and tumor margins during surgery. Specifically, we show that optical imaging probes topically applied to tumors and surrounding normal tissue rapidly differentiate between tissues. In contrast to systemic delivery of optical imaging probes which label tumors uniformly over time, topical probe application results in rapid and robust probe activation that is detectable as early as 5 minutes following application. Importantly, labeling is primarily associated with peri-tumor spaces. This methodology provides a means for rapid visualization of tumor and potentially infiltrating tumor cells and has potential applications for directed surgical excision of tumor tissues. Furthermore, this technology could find use in surgical resections for any tumors having differential regulation of cysteine cathepsin activity.