Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L
Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L
复制标题
联合QTL作图和转录组测序分析揭示了甘蓝型油菜的候选开花时间基因
DOI：
10.1186/s12864-018-5356-8
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发表时间：
2019-01-09
期刊：
影响因子：
4.4
通讯作者：
Liu, Liezhao
中科院分区：
文献类型：
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作者：
Jian, Hongju;Zhang, Aoxiang;Liu, Liezhao
背景：最佳开花期是甘蓝型油菜的重要农艺性状。为了研究这一重要作物开花时间的遗传结构和遗传规律，我们对6个环境中开花时间差异极大的重组自交系群体进行了开花时间的QTL分析，并进行了RNA-Seq分析。结果：我们在6个环境中检测到分布在8条染色体上的27个QTL，其中位于C02染色体上的一个主效QTL解释了11-25%的表型变异，并且在所有6个环境中都稳定地检测到。RNA-Seq分析发现了105个与开花时间相关的差异表达基因(DEG)，它们在生物钟/光周期、自主途径、激素和春化途径中发挥作用。结论：我们在这些QTL区域发现了45个与开花时间相关的基因，其中8个是DGs，其中包括花期调控基因伪反应调节基因7(PRR7)和花期调控基因FY(位于C02上的一个主效QTL区域)。这些发现为甘蓝型油菜开花时间的遗传结构提供了洞察力。
Background:Optimum flowering time is a key agronomic trait in Brassica napus. To investigate the genetic architecture and genetic regulation of flowering time in this important crop, we conducted quantitative trait loci (QTL) analysis of flowering time in a recombinant inbred line (RIL) population, including lines with extreme differences in flowering time, in six environments, along with RNA-Seq analysis.Results:We detected 27 QTLs distributed on eight chromosomes among six environments, including one major QTL on chromosome C02 that explained 11-25% of the phenotypic variation and was stably detected in all six environments. RNA-Seq analysis revealed 105 flowering time-related differentially expressed genes (DEGs) that play roles in the circadian clock/photoperiod, autonomous pathway, and hormone and vernalization pathways. We focused on DEGs related to the regulation of flowering time, especially DEGs in QTL regions.Conclusions:We identified 45 flowering time-related genes in these QTL regions, eight of which are DEGs, including key flowering time genes PSEUDO RESPONSE REGULATOR 7 (PRR7) and FY (located in a major QTL region on C02). These findings provide insights into the genetic architecture of flowering time in B. napus.