Molecular composition of nonionic vesicles prepared from Span 80 or Span 85 by a two-step emulsification method
Molecular composition of nonionic vesicles prepared from Span 80 or Span 85 by a two-step emulsification method
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DOI：
10.1080/01932690600859903
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Sugahara, Takuya
中科院分区：
文献类型：
--
作者：
Kato, Keiichi;Walde, Peter;Sugahara, Takuya
采用反相高效液相色谱法分析了商品非离子表面活性剂Span 80和Span 85的分子组成。两种表面活性剂都是脂肪酸酯的混合物，含有单酯、二酯、三酯和四酯。在Span 80中，二酯占主导地位，而Span 85主要含有四酯。从Span 80（或Span 85）中通过两步乳化方法制备囊泡，其中包括均质和分离步骤，其中一部分表面活性剂被去除。用高效液相色谱法分析了不同酯类化合物的组成。虽然商用Span 80和Span 85在分子组成上有很大的不同，但在两种情况下形成的囊泡的酯谱相当相似，并且以二酯为主。因此，特定的囊泡制备方法导致主要是那些易于形成双分子层的组分的分子选择。
The molecular compositions of the commercial nonionic surfactants Span 80 and Span 85 were analyzed by reversed phase high performance liquid chromatography (HPLC). Both surfactants are mixtures of fatty acid esters, containing monoesters, diesters, triesters, and tetraesters. While diesters dominate in the case of Span 80, Span 85 contains mainly tetraesters. Vesicles were prepared from Span 80 (or Span 85) by a two-step emulsification method that involved homogenization and separation steps in which a portion of the surfactants was removed. The composition of the vesicles was analyzed by HPLC with respect to the different esters present. Although commercial Span 80 and Span 85 differ considerably in their molecular compositions, the ester profiles of the vesicles formed were in both cases rather similar and dominated by diesters. Therefore, the particular vesicle preparation method leads to a molecular selection of mainly those components that are prone to form bilayers.