Overexpresssion of a Legionella pneumophila homologue of the E-coli regulator csrA affects cell size, flagellation, and pigmentation
Overexpresssion of a Legionella pneumophila homologue of the E-coli regulator csrA affects cell size, flagellation, and pigmentation
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DOI：
10.1078/1438-4221-00141
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发表时间：
2001-11-01
影响因子：
4.1
通讯作者：
Marre, R
中科院分区：
文献类型：
--
作者：
Fettes, PS;Forsbach-Birk, V;Marre, R
嗜肺军团菌是水生环境的居民，是细菌性肺炎的病原体。我们发现了一个L pneumophila同源物。大肠杆菌的rsmA和胡萝卜软腐欧文氏菌的rsmA，这些基因通过使mRNA不稳定来调节基因表达，并且已经显示与环境适应性和致病性有关。军团菌csrA能与大肠杆菌csrA阴性突变株互补。杆菌在L.嗜肺菌导致鞭毛形成和色素沉着减少以及细菌细胞大小增加。csrA的过度产生与fliA和flaA转录物的减少有关。这表明，与E. coli和欧文氏菌（Erwinia）、L.嗜肺菌csrA是基因表达的调节因子，可能有助于病原体快速适应变化的环境的能力。
Legionella pneumophila is an inhabitant of the aquatic environment and the causative agent of a bacterial pneumonia. We identified the presence of an L. pneumophila homologue of csrA of E. coli and rsmA of Erwinia carotovora, genes which regulate gene expression by destabilising mRNA and which have been shown to relate to environmental fitness and pathogenicity. The Legionella csrA was able to complement a csrA-negative mutant of E. coli. Overproduction of csrA in L. pneumophila lead to a reduction of flagellation and pigmentation and an increase in bacterial cell size. csrA overproduction was associated with a reduction of fliA and flaA transcripts. This suggests that similar to E. coli and Erwinia, L. pneumophila csrA is a regulator of gene expression and may contribute to the capability of the pathogen to rapidly adapt to changing environments.