VARICELLA-ZOSTER VIRUS (VZV) OPEN READING FRAME-10 PROTEIN, THE HOMOLOG OF THE ESSENTIAL HERPES-SIMPLEX VIRUS PROTEIN-VP16, IS DISPENSABLE FOR VZV REPLICATION IN-VITRO
VARICELLA-ZOSTER VIRUS (VZV) OPEN READING FRAME-10 PROTEIN, THE HOMOLOG OF THE ESSENTIAL HERPES-SIMPLEX VIRUS PROTEIN-VP16, IS DISPENSABLE FOR VZV REPLICATION IN-VITRO
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DOI：
10.1128/jvi.68.12.7850-7858.1994
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发表时间：
1994-12-01
影响因子：
5.4
通讯作者：
SEIDEL, K
中科院分区：
文献类型：
--
作者：
COHEN, JI;SEIDEL, K
水痘带状疱疹病毒（VZV）开放阅读框10（ORF 10）蛋白是单纯疱疹病毒1型（HSV-1）蛋白VP 16的同源物。VZV ORF 10反式激活VZV IE62基因，并且是存在于病毒粒子中的被膜蛋白。HSV-1VP 16是HSV-1立即早期基因和被膜蛋白的有效反式激活因子，对HSV-1的体外复制至关重要。为了确定VZV ORF 10是否是体外病毒复制所必需的，我们构建了两个不能表达ORF 10的VZV突变体。一个突变体在ORF 10基因的第61个密码子后有一个终止密码子，另一个突变体除了基因的最后5个密码子外，其余密码子都被删除。两种VZV突变体在细胞培养物中生长至与亲本病毒相似的滴度。为了确定HSV-1 VP 16是否改变VZV的生长，我们构建了一个VZV突变体，其中VP 16插入ORF 10的位置。免疫电镜观察发现，重组VZV病毒粒子被膜中存在HSV-1VP 16。VZV VP 16置换突变体产生的噬斑较小，并且生长至比亲本病毒更低的滴度。因此，VZV ORF 10不是病毒体外生长所必需的，并且HSV-1 VP 16取代VZV ORF 10会损害VZV的生长。
Varicella-zoster virus (VZV) open reading frame 10 (ORF10) protein is the homolog of the herpes simplex virus type 1 (HSV-1) protein VP16. VZV ORF10 transactivates the VZV IE62 gene and is a tegument protein present in the virion. HSV-1 VP16, a potent transactivator of HSV-1 immediate early genes and tegument protein, is essential for HSV-1 replication in vitro. To determine whether VZV ORF10 is required for viral replication in vitro, we constructed two VZV mutants which were unable to express ORF10. One mutant had a stop codon after the 61st codon of the ORF10 gene, and the other mutant was deleted for all but the last five codons of the gene. Both VZV mutants grew in cell culture to titers similar to that of the parental virus. To determine whether HSV-1 VP16 alters the growth of VZV, we constructed a VZV mutant in which VP16 was inserted in place of ORF10. Using immune electron microscopy, we found that HSV-1 VP16 was present in the tegument of the recombinant VZV virions. The VZV VP16 substitution mutant produced smaller plaques and grew to a lower titer than parental virus. Thus, VZV ORF10 is not required for growth of the virus in vitro, and substitution of HSV-1 VP16 for VZV ORF10 impairs the growth of VZV.