High performance liquid chromatographic assay for the quantitation of total glutathione in plasma
High performance liquid chromatographic assay for the quantitation of total glutathione in plasma
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DOI：
10.1016/s0731-7085(01)00658-6
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发表时间：
2002-05-15
影响因子：
3.4
通讯作者：
Ganafa, AA
中科院分区：
文献类型：
--
作者：
Abukhalaf, IK;Silvestrov, NA;Ganafa, AA
建立了一种简便、应用广泛的同型半胱氨酸高效液相色谱(HPLC法)测定血浆中谷胱甘肽的方法。该方法以SBDF为衍生化试剂，仅需50微升的进样量。谷胱甘肽在0.3125-62.50Mumol/L浓度范围内呈线性关系，标准曲线的线性回归分析相关系数为0.999。最低检出限(LOD)和定量限(LOQ)值分别为5.0和15pmol。用该方法回收谷胱甘肽接近完成(96%以上)。批内和批间精密度研究反映了该方法的高度可靠性和重复性。用人和大鼠血浆样品验证了该方法对谷胱甘肽定量的适用性。(C)2002 Elsevier Science B.V.保留所有权利。
A simple and widely used homocysteine HPLC procedure was applied for the HPLC identification and quantitation of glutathione in plasma. The method, which utilizes SBDF as a derivatizing agent utilizes only 50 mul of sample volume. Linear quantitative response curve was generated for glutathione over a concentration range of 0.3125-62.50 mumol/l. Linear regression analysis of the standard curve exhibited correlation coefficient of 0.999. Limit of detection (LOD) and limit of quantitation (LOQ) values were 5.0 and 15 pmol, respectively. Glutathione recovery using this method was nearly complete (above 96%). Intra-assay and inter-assay precision studies reflected a high level of reliability and reproducibility of the method. The applicability of the method for the quantitation of glutathione was demonstrated successfully using human and rat plasma samples. (C) 2002 Elsevier Science B.V. All rights reserved.