Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis.
Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis.
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拟南芥小核仁 RNA 监测核糖体生物发生过程中前 rRNA 的有效加工。
DOI：
10.1073/pnas.1614852113
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发表时间：
2016
影响因子：
11.1
通讯作者：
Zhu Danmeng
中科院分区：
文献类型：
--
作者：
Zhu Pan;Wang Yuqiu;Qin Nanxun;Wang Feng;Wang Jia;Deng Xing Wang;Zhu Danmeng
真核生物中核糖体的产生需要数百个组装因子的复杂和精确的协调，包括许多小核仁RNA（snoRNA）。然而，目前，在高等植物中的核糖体生物合成的关键snoRNA的独特作用仍然知之甚少。在这里，我们报告了一个以前未知的C（RUGAUGA）/D（CUGA）型snoRNA，隐藏的宝藏2（HID 2），作为一个重要的调节核糖体生物合成通过snoRNA-rRNA相互作用。核定位的HID 2在增生性组织中表达活跃，而HID 2的缺陷会导致一系列发育缺陷，使人联想到核糖体蛋白突变体。HID 2与前体45 S rRNA结合，促进前体rRNA加工的效率和准确性。有趣的是，破坏HID 2蛋白似乎会损害27 SB的完整性，27 SB是一种关键的前rRNA中间体，可以产生25 S和5.8S rRNA，已知对60 S核糖体大亚基的合成至关重要，也会产生不平衡的核糖体谱。最后，我们证明了HID 2的反义框在真核生物中是功能必需的和高度保守的。总的来说，我们的研究揭示了snoRNA在核糖体生物合成过程中监测前rRNA加工效率的重要和可能保守的作用。
Ribosome production in eukaryotes requires the complex and precise coordination of several hundred assembly factors, including many small nucleolar RNAs (snoRNAs). However, at present, the distinct role of key snoRNAs in ribosome biogenesis remains poorly understood in higher plants. Here we report that a previously uncharacterized C (RUGAUGA)/D (CUGA) type snoRNA,HIDDEN TREASURE 2(HID2), acts as an important regulator of ribosome biogenesis through a snoRNA–rRNA interaction. Nucleolus-localizedHID2is actively expressed inArabidopsisproliferative tissues, whereas defects inHID2cause a series of developmental defects reminiscent of ribosomal protein mutants.HID2associates with the precursor 45S rRNA and promotes the efficiency and accuracy of pre-rRNA processing. Intriguingly, disruptingHID2inArabidopsisappears to impair the integrity of 27SB, a key pre-rRNA intermediate that generates 25S and 5.8S rRNA and is known to be vital for the synthesis of the 60S large ribosomal subunit and also produces an imbalanced ribosome profile. Finally, we demonstrate that the antisense-box ofHID2is both functionally essential and highly conserved in eukaryotes. Overall, our study reveals the vital and possibly conserved role of a snoRNA in monitoring the efficiency of pre-rRNA processing during ribosome biogenesis.