Expression of replication-dependent histone genes in avian spermatids involves an alternate pathway of mRNA 3'-end formation.
Expression of replication-dependent histone genes in avian spermatids involves an alternate pathway of mRNA 3'-end formation.
复制标题
鸟类精细胞中复制依赖性组蛋白基因的表达涉及 mRNA 3 端形成的替代途径。
DOI：
10.1128/mcb.9.3.902-913.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Groudine,M
中科院分区：
文献类型：
--
作者：
Challoner,PB;Moss,SB;Groudine,M
In somatic cells the expression of replication-dependent histone genes is coupled to the S phase of the cell cycle. However, we have found a number of novel H2a, H2b, and H3 poly(A)+RNA species in avian haploid round spermatids. The spermatid-specific H2a and H2b 0.8-kilobase RNAs are transcribed from a subset of the replication-dependent H2a and H2b gene families. Two cDNAs derived from the spermatid-specific H2b transcripts were isolated and sequenced. The structures of these cDNAs reveal that the spermatid-specific RNAs are identical to the 0.5-kilobase poly(A)−H2b mRNAs expressed in proliferating somatic cells, except for the addition of poly(A) at the 3′ ends. The site of poly(A) addition in the spermatid-specific RNAs is located 26 to 28 nucleotides 3′ of the poly(A)−H2b mRNA terminus. Thus, the hairpin structures and purine-rich elements required for the U7 small nuclear ribonucleoprotein-mediated cleavage reaction that generates the 3′ ends of poly(A)−H2b mRNAs are not utilized in spermatids and are retained in the poly(A)+H2b RNAs.
