Expression of ribosomal-protein genes in Xenopus laevis development
Expression of ribosomal-protein genes in Xenopus laevis development
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非洲爪蟾发育中核糖体蛋白基因的表达
DOI：
10.1016/0092-8674(82)90022-8
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
F. Amaldi
中科院分区：
文献类型：
--
作者：
P. Pierandrei;N. Campioni;E. Beccari;I. Bozzoni;F. Amaldi
使用探针非洲爪蟾核糖体蛋白（R-蛋白）mRNA，我们已经发现，在卵母细胞中的R-蛋白mRNA的积累进行到最高水平，这是在卵黄发生的开始，并保持稳定。在胚胎中，r蛋白mRNA序列在早期卵裂（第24阶段）期间以低水平存在于细胞质中，直到原肠胚形成（第10阶段）才变得不可检测，并且之后逐渐积累。mRNA量与细胞数量的归一化表明在第10阶段左右R蛋白基因的激活;然而，不能排除mRNA周转率的变化。新合成的核糖体蛋白从早期裂解到第26阶段不能被发现，除了S3、L17和L31，它们是不断产生的，以及蛋白质L5，它在第7阶段左右开始合成。一套完整的核糖体蛋白仅在尾芽胚胎（阶段28-32）中活跃地产生，在它们的mRNA出现后几个小时。在第26阶段之前，这些mRNA序列被发现在亚多核糖体组分上，而在第31阶段，它们中的50%以上与多核糖体相关。无核突变体不合成核糖体蛋白质的时候，正常胚胎非常活跃，但他们积累的r-蛋白mRNA。
Using probes to Xenopus laevis ribosomal-protein (r-protein) mRNAs, we have found that in the oocyte the accumulation of r-protein mRNAs proceeds to a maximum level, which is attained at the onset of vitellogenesis and remains stable thereafter. In the embryo, r-protein mRNA sequences are present at low levels in the cytoplasm during early cleavage (stages 24, become undetectable until gastrulation (stage 10) and accumulate progressively afterwards. Normalization of the amount of mRNA to cell number suggests an activation of r-protein genes around stage 10; however, a variation in mRNA turnover cannot be excluded. Newly synthesized ribosomal proteins cannot be found from early cleavage up to stage 26, with the exception of S3, L17 and L31, which are constantly made, and protein L5, which starts to be synthesized around stage 7. A complete set of ribosomal proteins is actively produced only in tailbud embryos (stages 28-32), several hours after the appearance of their mRNAs. Before stage 26 these mRNA sequences are found on subpolysomal fractions, whereas more than 50% of them are associated with polysomes at stage 31. Anucleolate mutants do not synthesize ribosomal proteins at the time when normal embryos do it very actively; nevertheless, they accumulate r-protein mRNAs.
非洲爪蟾卵母细胞和体细胞中相关的 5S RNA 转录因子。
DOI：
10.1073/pnas.78.3.1760
发表时间：
1981
影响因子：
11.1
作者：
Pelham,HR;Wormington,WM;Brown,DD
通讯作者：
Brown,DD