Neutralizing Antibodies Limit Cell-Associated Spread of Human Cytomegalovirus in Epithelial Cells and Fibroblasts.
Neutralizing Antibodies Limit Cell-Associated Spread of Human Cytomegalovirus in Epithelial Cells and Fibroblasts.
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DOI：
10.3390/v14020284
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发表时间：
2022-01-28
期刊：
影响因子：
--
通讯作者：
Thomas M
中科院分区：
文献类型：
--
作者：
Reuter N;Kropff B;Britt WJ;Mach M;Thomas M
Human cytomegalovirus (HCMV) can cause severe clinical disease in immunocompromised individuals, such as allograft recipients and infants infected in utero. Neutralizing activity of antibodies, measured as the ability to prevent the entry of cell-free virus, has been correlated with the reduction in HCMV transmission and the severity of HCMV-associated disease. However, in vivo HCMV amplification may occur mainly via cell-to-cell spread. Thus, quantifying the inhibition of cell-to-cell transmission could be important in the evaluation of therapeutic antibodies and/or humoral responses to infection or immunization. Here, we established a quantitative plaque reduction assay, which allowed for the measurement of the capacity of antibodies to limit HCMV spread in vitro. Using an automated fluorescence spot reader, infection progression was assayed by the expansion of viral plaques during the course of infection with various GFP-expressing viruses. We found that in contrast to non-neutralizing monoclonal antibodies (mAbs), neutralizing mAbs against both glycoprotein B and H (gB and gH) could significantly inhibit viral plaque expansion of different HCMV strains and was equally efficient in fibroblasts as in epithelial cells. In contrast, an anti-pentamer mAb was active only in epithelial cells. Taken together, our data demonstrate that specific anti-HCMV mAbs can significantly limit cell-associated virus spread in vitro.
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影响因子：
6.7
作者：
Auerbach MR;Yan D;Vij R;Hongo JA;Nakamura G;Vernes JM;Meng YG;Lein S;Chan P;Ross J;Carano R;Deng R;Lewin-Koh N;Xu M;Feierbach B
通讯作者：
Feierbach B
DOI：
10.3390/v7122957
发表时间：
2015-12-11
期刊：
Viruses
影响因子：
--
作者：
Cooper RS;Heldwein EE
通讯作者：
Heldwein EE
影响因子：
5.4
作者：
Chiuppesi, Flavia;Wussow, Felix;Diamond, Don J.
通讯作者：
Diamond, Don J.
DOI：
10.1073/pnas.1818130116
发表时间：
2019-03-19
影响因子：
11.1
作者：
Cudini, Juliana;Roy, Sunando;Breuer, Judith
通讯作者：
Breuer, Judith
影响因子：
5.4
作者：
BRITT, WJ;VUGLER, LG
通讯作者：
VUGLER, LG