Effects of intracavernous administration of adrenomedullin on erectile function in rats
Effects of intracavernous administration of adrenomedullin on erectile function in rats
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DOI：
10.1016/s0196-9781(01)00521-6
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发表时间：
2001-11-01
期刊：
影响因子：
3
通讯作者：
Kitamura, T
中科院分区：
文献类型：
--
作者：
Nishimatsu, H;Hirata, Y;Kitamura, T
我们已经报道，肾上腺髓质素（AM）诱导的血管舒张至少部分是一氧化氮（NO）-cGMP依赖性的大鼠。虽然NO在勃起功能中的作用已为人们所熟知，但AM是否影响勃起功能仍存在争议。因此，我们研究了AM对阴茎勃起期间海绵体内压（ICP）的影响。大鼠左颈总动脉插管，监测平均动脉压（MAP）。双极电极定位在海绵体神经上。用连接到压力传感器的针插入右侧海绵体以监测ICP。电刺激（ES）以电压依赖性方式增加ICP。ES期间ICP持续升高。海绵体内注射0.5 nmol AM显著增强ES诱导的最大ICP/MAP和曲线下面积（ICP描记; AUC）的增加。由于AM略微降低MAP，因此通过MAP使ICP正常化。静脉注射NO合成酶抑制剂N-ω-硝基-L-精氨酸可显著降低AM/ES诱导的ICP升高。然而，在存在eGMP特异性磷酸二酯酶抑制剂E-4021的情况下，AM进一步增加ICP/MAP和AUC。这些结果表明，一个NO-cGMP途径参与调节AM诱导的大鼠海绵体血管舒张。(C)2001 Elsevier Science Inc. All rights reserved.
We have reported that adrenomedullin (AM)-induced vasodilation is at least in part nitric oxide (NO)-cGMP-dependent in the rat. Although it is well known that NO is much involved in the erectile function, it is controversial as to whether AM influences the erectile function. Thus, we examined the effects of AM on intracavernous pressure (ICP) during penile erection. The left carotid artery of rats was cannulated to monitor of mean arterial pressure (MAP). Bipolar electrodes were positioned on the cavernous nerve. The right cavernous body was cannulated with a needle connected to a pressure transducer to monitor ICP. Electrical stimulation (ES) increased ICP in a voltage-dependent manner. Elevation of ICP continued during ES. The intracavernous injection of 0.5 nmol AM significantly potentiated ES-induced increases in both maximal developed ICP/MAP and area under the curve (ICP trace; AUC). Since AM slightly lowered MAP, ICP was normalized by MAP. i.v. administration of N-omega-nitro-L-arginine, a NO synthase inhibitor, markedly decreased AM/ES-induced ICP elevation. However, in the presence of E-4021, a eGMP-specific phosphodiesterase inhibitor, AM further increased both ICP/MAP and AUC. These results suggest that a NO-cGMP pathway is involved in the regulation of AM-induced rat cavernous vasorelaxation. (C) 2001 Elsevier Science Inc. All rights reserved.