Human embryonic stem cell lines with genetic disorders
Human embryonic stem cell lines with genetic disorders
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DOI：
10.1016/s1472-6483(10)60810-3
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Kuliev, A
中科院分区：
文献类型：
--
作者：
Verlinsky, Y;Strelchenko, N;Kuliev, A
先前的一项研究描述了从不同来源的胚胎材料中建立人类胚胎干细胞（ESC）系。包括桑葚胚、整个囊胚和分离的内细胞团。利用这些方法，建立了具有不同遗传异常的ESC细胞系库，为研究遗传异常细胞中细胞过程的主要干扰提供了无限的疾病细胞培养来源。在植入前遗传学诊断（PGD）的持续实践中，遗传疾病的ESC系来源于检测到的突变胚胎，并避免了移植。目前的存储库包含有遗传疾病的IS ESC系。包括罹。杜氏和贝克尔肌营养不良症、范可尼贫血、补体a族脆弱- x综合征、亨廷顿病（三条系）、马凡氏综合征、肌强直性营养不良症（两条系）、I型神经纤维瘤病（五条系）和地中海贫血（两条系）。这些ESC线目前用于研究目的，并可根据要求提供。
A previous study described the establishment of human embryonic stem cell (ESC) lines from different sources of embryonic material. including morula, whole blastocyst and isolated inner cell mass. Using these methods, a repository of ESC lines has been established with different genetic abnormalities, which provides an unlimited source of disease cells in culture for undertaking research oil the primary disturbances of the cellular processes in the genetically abnormal cells. ESC lines with genetic disorders were derived from the mutant embryos detected and avoided from transfer in the ongoing practice of preimplantation genetic diagnosis (PGD). The current repository contains IS ESC lines with genetic disorders. including adrenoleukodystrophy. Duchenne and Becker muscular dystrophy, Fanconi anaemia, complementation group A. fragile-X syndrome, Huntington disease (three lines), Marfan syndrome, myotonic dystrophy (two lines), neurofibromatosis type I (five lines) and thalassaemia (two lines). These ESC lines are presently used for research purposes and may be available on request.