Endogenous 5‐HT inhibits firing activity of hippocampal CA1 pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT1A receptors
Endogenous 5‐HT inhibits firing activity of hippocampal CA1 pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT1A receptors
复制标题
DOI：
10.1002/hipo.10178
复制
发表时间：
2004
期刊：
影响因子：
3.5
通讯作者：
K. Tada;K. Kasamo;Tadashi Suzuki;Y. Matsuzaki;T. Kojima
中科院分区：
文献类型：
--
作者：
K. Tada;K. Kasamo;Tadashi Suzuki;Y. Matsuzaki;T. Kojima
本研究检测了在条件恐惧应激(CFS)诱导的冰冻行为中，未麻醉的、自由的大鼠海马区CA1区锥体神经元的活动。条件性冰冻行为使大鼠海马CA1区锥体神经元放电频率明显降低。在冻结行为消失后，放电频率恢复到基线。选择性5-羟色胺(5-HT)1A拮抗剂N‐[2‐[4‐(2‐methoxyphenyl)‐1‐piperazinyl]ethyl]‐N‐(2‐pyridinyl)cyclohexanecarboxamide(WAY-100635)和N‐tert‐butyl‐3‐[4‐(2‐methoxyphenyl)piperazine‐1‐yl]‐2‐phenylpropamide(WAY-100135)和5-羟色胺与对氯苯丙氨酸(PCPA)一起耗竭可完全消除CFs诱导的冰冻行为中放电频率的降低。这些结果提示，内源性5-羟色胺主要通过兴奋5-HT1a受体来抑制CFS诱导的冰冻行为中海马CA1区锥体神经元的放电活动。©2004 Wiley-Liss Inc.
This study examines the activity of hippocampal CA1 pyramidal neurons during conditioned fear stress (CFS)‐induced freezing behavior in unanesthetized, unrestrained rats. The firing frequency of hippocampal CA1 pyramidal neurons was significantly decreased when conditioned rats exhibited freezing behavior. Firing frequency returned to the baseline after freezing behavior disappeared. The selective 5‐hydroxytryptamine (5‐HT)1A antagonists, N‐[2‐[4‐(2‐methoxyphenyl)‐1‐piperazinyl]ethyl]‐N‐(2‐pyridinyl)cyclohexanecarboxamide (WAY‐100635), and N‐tert‐butyl‐3‐[4‐(2‐methoxyphenyl)piperazine‐1‐yl]‐2‐phenylpropamide (WAY‐100135) and 5‐HT depletion with parachlorophenylalanine (PCPA) completely abolished the decrease in firing frequency during CFS‐induced freezing behavior. These results suggested that endogenous 5‐HT inhibited the firing activity of hippocampal CA1 pyramidal neurons during CFS‐induced freezing behavior mainly through stimulating 5‐HT1A receptors. © 2004 Wiley‐Liss, Inc.