A new member of the PBAN family in Spodoptera littoralis:: molecular cloning and immunovisualisation in scotophase hemolymph
A new member of the PBAN family in Spodoptera littoralis:: molecular cloning and immunovisualisation in scotophase hemolymph
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DOI：
10.1016/s0965-1748(01)00179-5
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发表时间：
2002-08-01
影响因子：
3.8
通讯作者：
Jacquin-Joly, E
中科院分区：
文献类型：
--
作者：
Iglesias, F;Marco, P;Jacquin-Joly, E
在这篇文章中，我们报告的证据表明，以前在Spodoptera littoralis暗期血淋巴中检测到的免疫反应因子是PBAN，这支持激素的体液途径的信息素腺体。将预纯化的暗期血淋巴提取物进行非变性PAGE后，进行Western blot，得到一条与S. littoralis Br-SOG因子和预期的移动性为一个arctuid PBAN。该条带在嗜水气单胞菌血淋巴提取物中未检出。S.利用RT-PCR技术克隆了一个滨海小球藻Br-SOG因子的cDNA片段。这使我们能够推断出Spl-PBAN的氨基酸序列，其与其他已知的PBAN高度同源。此外，我们发现PBAN编码cDNA还编码其他四个推定的酰胺化肽（Spl-DH同源物，Spl-alpha-NP，Spl-beta-NP和Spl-gamma-NP），它们在拟南芥中是相同的或高度保守的，以及两个未知功能的非酰胺化肽。这种cDNA组织对于所有已知的编码PBAN的cDNA是共同的，导致在假定的前激素原酶促裂解后释放不同的肽。(C)2002爱思唯尔科技有限公司版权所有。
In this article, we report evidence suggesting that the immunoreactive factor previously detected in Spodoptera littoralis scotophase hemolymph is PBAN, which supports a humoral route of the hormone to the pheromone gland. Western blot after native-PAGE of prepurified scotophase hemolymph extracts yielded an immunoreactive band with the same mobility as S. littoralis Br-SOG factor and the expected mobility for a noctuid PBAN. This band was not detected in photophase hemolymph extract. The identity of S. littoralis Br-SOG factor as PBAN was obtained from cDNA cloning using RT-PCR strategy. This allowed us to deduce the amino acid sequence of Spl-PBAN, which is highly homologous to other known PBANs. Moreover, we found that the PBAN encoding cDNA also encoded four other putative amidated peptides (Spl-DH homologue, Spl-alpha-NP, Spl-beta-NP and Spl-gamma-NP) that are identical or highly conserved among noctuids, and two non amidated peptides of unknown function. This cDNA organization is common to all known cDNAs encoding PBANs, leading to the release of different peptides after putative enzymatic cleavage of the preprohormone. (C) 2002 Elsevier Science Ltd. All rights reserved.