The tryptic and chymotryptic fragments of the β‐subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin

The tryptic and chymotryptic fragments of the β‐subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin
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脑中鸟嘌呤核苷酸结合蛋白β亚基的胰蛋白酶和胰凝乳蛋白酶片段与视网膜转导蛋白的片段相同

DOI:
10.1016/0014-5793(85)80332-x
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发表时间:
1985
期刊:
影响因子:
3.5
通讯作者:
A. Spiegel
A. Spiegel
中科院分区:
生物学3区
文献类型:
--
作者:
M. Pines;P. Gierschik;A. Spiegel

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从杆状细胞外节膜中纯化的转导蛋白35 - kDa β -亚基被胰蛋白酶裂解为2个主要片段,被胰凝乳胰蛋白酶裂解为7个主要片段。在棒材外段膜、纯化的脑鸟嘌呤核苷酸结合蛋白和脑膜蛋白水解后,用转导素β特异性抗血清进行免疫印迹观察相同的片段。结果表明,转导蛋白和脑鸟嘌呤核苷酸结合蛋白的β‐亚基不仅在结构上相似,而且在蛋白水解酶的可及性方面在膜上的取向也相似。
The 35‐kDa β‐subunit of transducin purified from rod outer segment membranes is cleaved into 2 major fragments by trypsin, and 7 major fragments by chymotrypsin. Identical fragments are visualized by immunoblotting with transducin‐β specific antisera after proteolysis of rod outer segment membranes, purified brain guanine nucleotide binding proteins, and brain membranes. The results indicate that the β‐subunits of transducin and of brain guanine nucleotide binding proteins are not only similar structurally, but are also similarly oriented in membranes with respect to accessibility to proteolytic enzymes.
DOI: --
发表时间: 1984
期刊: The Journal of biological chemistry
影响因子: --
作者:
Halliday,KR;Stein,PJ;Chernoff,N;Wheeler,GL;Bitensky,MW
通讯作者: Bitensky,MW
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
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通讯作者: Gilman,AG
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发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
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DOI: 10.1016/s0021-9258(18)89817-9
发表时间: 1984-11
期刊: The Journal of biological chemistry
影响因子: --
作者:
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通讯作者: P. Sternweis;J. Robishaw
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
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通讯作者: Nash,CR