Aedes aegypti microRNA miR-2b regulates ubiquitin-related modifier to control chikungunya virus replication.
Aedes aegypti microRNA miR-2b regulates ubiquitin-related modifier to control chikungunya virus replication.
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DOI：
10.1038/s41598-017-18043-0
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发表时间：
2017-12-15
影响因子：
4.6
通讯作者：
Sunil S
中科院分区：
文献类型：
--
作者：
Dubey SK;Shrinet J;Jain J;Ali S;Sunil S
在蚊子体内复制的虫媒病毒激活蚊子体内的先天免疫反应。已知在基孔肯雅病毒感染期间调节性非编码microRNA（miRNA）在蚊子中被调节。然而，关于这些miRNAs的靶点的信息很少。本研究旨在鉴定和分析基孔肯雅病毒（CHIKV）在埃及伊蚊细胞和蚊子中复制过程中调控的miRNAs的靶标。采用新一代测序技术，我们从Ae中鉴定了总共126个miRNAs。埃及伊蚊细胞株Aag 2.其中，发现13种miRNA在CHIKV感染期间受到调控。在细胞系和蚊子中，还使用定量PCR分析了三种最显著调节的miRNAs- miR-100、miR-2b和miR-989的推定靶标，以验证它们是否是miRNAs的靶标。我们的研究扩展了Ae中已知的miRNAs列表。埃及和预测的目标显着调节的miRNA。对其中一些靶点的进一步分析表明，泛素相关修饰物是miRNA miR-2b的靶点，并在基孔肯雅病毒复制中起着重要作用。
Arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. Regulatory non-coding microRNAs (miRNA) are known to be modulated in mosquitoes during chikungunya infection. However, information about targets of these miRNAs is scant. The present study was aimed to identify and analyze targets of miRNAs that are regulated during chikungunya virus (CHIKV) replication in Aedes aegypti cells and in the mosquito. Employing next-generation sequencing technologies, we identified a total of 126 miRNAs from the Ae. aegypti cell line Aag2. Of these, 13 miRNAs were found to be regulated during CHIKV infection. Putative targets of three of the most significantly regulated miRNAs- miR-100, miR-2b and miR-989 were also analyzed using quantitative PCRs, in cell lines and in mosquitoes, to validate whether they were the targets of the miRNAs. Our study expanded the list of miRNAs known in Ae. aegypti and predicted targets for the significantly regulated miRNAs. Further analysis of some of these targets revealed that ubiquitin-related modifier is a target of miRNA miR-2b and plays a significant role in chikungunya replication.