ASPARTATE, GLUTAMATE AND GAMMA-AMINOBUTYRIC ACID DEPOLARIZE CULTURED ASTROCYTES
ASPARTATE, GLUTAMATE AND GAMMA-AMINOBUTYRIC ACID DEPOLARIZE CULTURED ASTROCYTES
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DOI：
10.1016/0304-3940(84)90345-8
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
--
作者：
KETTENMANN, H;BACKUS, KH;SCHACHNER, M
从出生后早期的大鼠大脑半球培养的分化的胶质纤维酸性蛋白阳性星形胶质细胞对谷氨酸、GABA和天冬氨酸有2- 36mv的去极化反应，但对甘氨酸或牛磺酸没有反应。虽然GABA导致短暂的去极化，但谷氨酸和天冬氨酸的作用在应用过程中持续存在。由于神经元不存在于这些培养中，因此可以排除来自相邻神经元的递质介导的K+释放的贡献。因此，由这些神经递质引发的去极化是星形胶质细胞的内在反应。
Cultures of differentiated, glial fibrillary acidic protein-positive astrocytes from early postnatal rat cerebral hemispheres respond with depolarization of 2-36 mV to glutamate, GABA and aspartate but not to glycine or taurine. While GABA resulted in a transient depolarization, the effect of glutamate and aspartate persisted during the application. Since neurons were not present in these cultures a contribution of transmitter-mediated K+ release from adjacent neurons could be excluded. The depolarization triggered by these neurotransmitters is therefore an intrinsic reaction of astrocytes.