Patterns of Endopolyploidy During Seedling Development in Cabbage ( Brassica oleracea L.).

Patterns of Endopolyploidy During Seedling Development in Cabbage ( Brassica oleracea L.).
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甘蓝(Brassica oleracea L.)幼苗发育过程中内多倍体的模式。

DOI:
10.1006/anbo.2000.1337
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发表时间:
2001
期刊:
影响因子:
4.2
通讯作者:
Y. Kimura
Y. Kimura
中科院分区:
生物学2区
文献类型:
--
作者:
N. Kudo;Y. Kimura

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对卷心菜 (Brassica oleracea L.) 体细胞核 DNA 含量的流式细胞术分析揭示了广泛的内多倍化,导致组织包含具有不同 DNA 含量(范围从 2C 到 16C)的细胞混合物。内多倍体的模式对于每个发育阶段都是特定的。干种子的胚中不存在多重多倍体。在种子萌发过程中,胚根和下胚轴发生快速内复制。在除茎尖外的所有组织中均检测到进一步的核内复制循环。在测试的五个卷心菜品种中,幼苗含有四种倍性水平的细胞,对应于2C、4C、8C和16C。多倍体可能是卷心菜植物分化程序的一个组成部分。讨论了卷心菜植物中核内复制的生物学意义。
Flow cytometric analysis of nuclear DNA contents of somatic tissues of cabbage ( Brassica oleracea L.) has revealed extensive endopolyploidization, resulting in tissues that comprise mixtures of cells with different DNA contents, ranging from 2C to 16C. Patterns of endopolyploidy are specific to each developmental stage. Multiple polyploidy was not present in the embryos of dry seeds. Rapid endoreduplication occurred in the radicle and the hypocotyl of the embryos during seed germination. Further endoreduplication cycles were detected in all tissues except those of the shoot tips. In five cabbage cultivars tested, seedlings contained cells of four ploidy levels, corresponding to 2C, 4C, 8C and 16C. Multiploidy may be an integral part of differentiation programs in cabbage plants. The biological significance of endoreduplication in cabbage plants is discussed.
DOI: 10.1105/tpc.5.11.1661
发表时间: 1993-11-01
期刊: PLANT CELL
影响因子: 11.6
作者:
MELARAGNO, JE;MEHROTRA, B;COLEMAN, AW
通讯作者: COLEMAN, AW