Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry
Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry
复制标题
使用纳米粒子辅助激光解吸/电离质谱法对植物激素进行成像
DOI：
10.1007/978-1-0716-2030-4_9
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发表时间：
2022
影响因子：
--
通讯作者：
Shiono Katsuhiro
中科院分区：
文献类型：
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作者：
Taira Shu;Shiono Katsuhiro
植物激素在生物和非生物胁迫及植物生长发育过程中具有协同和拮抗作用。因此，需要一种技术来同时测定几种植物激素的分布和浓度。质谱成像（MSI）是一种相对较新的技术，可以直接绘制和成像组织切片上的生物分子。MSI允许在单个植物组织切片上同时检测多种分析物，即使在缺乏目标特异性标记物（如抗体）的情况下。最近，MSI被用于通过纳米粒子辅助激光解吸/电离（Nano-PALDI）质谱（MS）方法来定位多种小分子（m/z< 500）植物激素。在这里，我们展示了一种使用Nano-PALDI MSI的多激素成像技术，并讨论了其在研究激素信号在植物发育和胁迫反应中的作用方面的潜力。
Plant hormones can act in synergistic and antagonistic ways in response to biotic and abiotic stresses and during plant growth and development. Thus, a technique is needed to simultaneously determine the distribution and concentration of several plant hormones. A relatively new technology, mass spectrometry imaging (MSI), enables the direct mapping and imaging of biomolecules on tissue sections. MSI permits simultaneous detection of multiple analytes on a single section of plant tissue, even in the absence of target-specific markers such as antibodies. Recently, MSI has been used to localize multiple, small molecule (m/z< 500) plant hormones by the nanoparticle-assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS) method. Here, we illustrate a technology for multiple-hormone imaging using Nano-PALDI MSI and discuss its potential in investigating the role of hormone signaling in plant development and stress responses.