Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sub-lethal concentrations of quaternium-15 in Mytilus galloprovincialis
Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sub-lethal concentrations of quaternium-15 in Mytilus galloprovincialis
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DOI：
10.1016/j.aquatox.2016.10.010
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发表时间：
2016-11-01
影响因子：
4.5
通讯作者：
Felice, Maria Rosa
中科院分区：
文献类型：
--
作者：
Faggio, Caterina;Pagano, Maria;Felice, Maria Rosa
环境中异生物质的存在通常会对生物体构成风险。季铵盐-15是一种防腐剂，是最常用的物质之一，被添加到几种化妆品和其他工业产品中。为此，结合海洋环境的生物指示剂，研究了该防腐剂对海洋无脊椎动物紫贻贝（Mytilus gallo-provincialis）的潜在毒理学效应。这项工作的结果证实，季铵盐-15，在0.1和1毫克/升浓度下使用，而代谢的M。galloprovincialis引起细胞活力的降低，以及防御和抗氧化系统的显著变化。事实上，血细胞活力显著降低，血淋巴参数测量表明动物受到压力。此外，自由基的产生，硫代巴比妥酸反应物质（TBARS）的浓度，并在热休克蛋白70的量增加，观察hepatopancreacreas.These变化表明，抗氧化系统被激活，压倒quatterabetrin-15诱导的氧化损伤。Quaternetes-15危害防御和抗氧化系统。这些结果提供了重要的信息与quattero-15在水生生物的生物命运，并确认生物标志物是现代环境评估的重要工具，因为它们可以帮助预测监测计划中涉及的污染物。(C)© 2016 Elsevier B. V.版权所有。
The presence of a xenobiotic in the environment can often represent a risk for living organisms. Quaternium-15, a preservative, is one of the most used substances and is added to several cosmetics and other industrial products. For this reason,kwowing the bio-indicator of the marine environment, the toxicological effects potentially elicited by this preservative on the marine invertebrate Mytilus gallo-provincialis were studied. The results of this work confirm that quaternium-15, used at 0.1 and 1 mg/l concentrations, while metabolized in M. galloprovincialis, causes a decrease in cellular viability, and remarkable changes to the defense and antioxidant system. In fact, haemocyte viability is dramatically reduced, and haemolymphatic parameter measurements indicate a stress on the animal. Moreover, an increase in radical species production, in Thiobarbituric Acid Reactive Species (TBARS) concentration, and in the Heat Shock Protein 70 amount, were observed in hepatopancreas.These changes suggest that the antioxidant systems are activated to overwhelm the oxidative damage induced by quaternium-15. Quaternium-15 jeopardizes both the defense and antioxidant systems. These results provide essential information with the biological fate of quaternium-15 in aquatic organisms, and confirm that biomarkers represent an important tool for modern environmental assessments as they can help with the prediction of pollutants involved in the monitoring program. (C) 2016 Elsevier B.V. All rights reserved.