New Genetic Variants of Anaplasma phagocytophilum and Anaplasma bovis from Korean Water Deer (Hydropotes inermis argyropus)
New Genetic Variants of Anaplasma phagocytophilum and Anaplasma bovis from Korean Water Deer (Hydropotes inermis argyropus)
复制标题
DOI：
10.1089/vbz.2010.0214
复制
发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Chae, Joon-seok
中科院分区：
文献类型：
--
作者：
Kang, Jun-gu;Ko, Sungjin;Chae, Joon-seok
野鹿是无形体属的重要天然储存宿主之一，其引起马、犬和人的粒细胞无形体病。本研究的目的是确定是否和什么种类的无形体自然感染韩国水鹿（KWD）在韩国。2008年3月至2009年5月期间，韩国野生动物保护基因组资源库共从KWD尸体上收集了66个脾脏。使用16S核糖体（r）RNA，用ankA、groEL和msp2基因引物进行聚合酶链反应（PCR）以扩增无形体属和埃里希体属的基因。用16SrRNA巢式PCR方法检测66份KWD脾脏中的嗜吞噬细胞无形体和牛无形体分别为42份（63.6%）和23份（34.8%）。42 A。嗜吞噬细胞A.序列分析将牛分为两个基因型。通过基于ankA、groEL和msp2的巢式PCR方法，对A.在66份样本中分别有1份（1.5%）、7份（10.6%）和3份（4.6%）检出嗜吞噬细胞菌。这些基因序列只有一种基因型。获得的7个16S rRNA基因序列中有5个从未被鉴定。所获得的ankA、groEL和msp2基因序列代表新的基因型。这是A. phagocytophilum和A. KWD中的牛，这表明它们可能作为无形体病人畜共患病病原体的水库。
Wild deer are one of the important natural reservoir hosts of Anaplasma species, which cause granulocytic anaplasmosis in equines, canines, and humans. The objective of the present study was to determine whether and what species of Anaplasma naturally infect Korean water deer (KWD) in the Republic of Korea. A total of 66 spleens from KWD carcasses were collected by the Conservation Genome Resource Bank for Korean Wildlife in Korea between March 2008 and May 2009. Polymerase chain reaction (PCR) was performed using 16S ribosomal (r) RNA, with ankA, groEL, and msp2 gene primers to amplify the genes of Anaplasma and Ehrlichia. Using 16S rRNA-based nested PCR, Anaplasma phagocytophilum and Anaplasma bovis were detected in 42 (63.6%) and 23 (34.8%) of 66 KWD spleens, respectively. The 42 A. phagocytophilum were classified into five genotypes and the 23 A. bovis were classified into two genotypes by sequence analysis. By ankA-, groEL-, and msp2-based nested PCR, A. phagocytophilum was detected in 1 (1.5%), 7 (10.6%), and 3 (4.6%) of 66 samples, respectively. These gene sequences had only one genotype. Five of seven obtained 16S rRNA gene sequences have never been identified. The ankA, groEL, and msp2 obtained gene sequences represented new genotypes. This is the first report of A. phagocytophilum and A. bovis in KWD, suggesting that they may act as reservoirs for anaplasmosis zoonotic pathogens.