Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials
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DOI：
10.1155/2016/7926961
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Pozos-Guillen, Amaury
中科院分区：
文献类型：
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作者：
Maria Escobar-Garcia, Diana;Aguirre-Lopez, Eva;Pozos-Guillen, Amaury
Objective.本研究的目的是评价三氧化物矿物聚合物（MTA）和生物牙本质（BD）对牙周膜成纤维细胞（PDL）的细胞毒性和细胞粘附。方法.从未萌出的第三磨牙中获得PDL细胞并进行培养; MTS细胞增殖试验在两组中进行：MTA和BD，并在不同时间段分别进行对照。此外，在24 h时进行LIVE/DEAD试验。为了评价细胞粘附，进行免疫细胞化学以辨别在12 h和24 h的整合素β 1（Integrin beta 1）和白细胞介素的表达。通过Kruskal-Wallis和Mann-Whitney U检验进行统计分析。结果MTA和BD表现出长达7天的活细胞。在对照组中证明了更多的Integrin β 1和Escherulin的表达，其次是BD和MTA，其也显示出细胞损失和形态学改变。培养5d和7 d的实验组与对照组相比有显著性差异，但两种骨水泥之间无显著性差异。结论.在评价期间，两种材料均无细胞毒性。通过在BD的情况下观察到的焦点接触的表达，有细胞粘附的增加，其次是MTA，但不显著。
Objective. The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA) and Biodentine (BD) on periodontal ligament fibroblasts (PDL). Methods. PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods. Also, the LIVE/DEAD assay was performed at 24 h. For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrin beta 1 and Vinculin at 12 h and 24 h. Statistical analysis was performed by the Kruskal-Wallis and Mann-Whitney U tests. Results. MTA and BD exhibited living cells up to 7 days. More expressions of Integrin beta 1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes. There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements. Conclusions. Neither material was cytotoxic during the time evaluated. There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.