Monoclonal antibody against non-histone chromosomal protein high mobility group 1 Co-migrates with high mobility group 1 into the nucleus.
Monoclonal antibody against non-histone chromosomal protein high mobility group 1 Co-migrates with high mobility group 1 into the nucleus.
复制标题
针对非组蛋白染色体蛋白高迁移率族 1 的单克隆抗体与高迁移率族 1 共同迁移到细胞核中。
DOI：
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发表时间：
1986
影响因子：
4.8
通讯作者：
T. Uchida
中科院分区：
文献类型：
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作者：
M. Tsuneoka;N. Imamoto;T. Uchida
当通过红细胞介导的显微注射注射到牛成纤维细胞和HeLa细胞的细胞质中时，非组蛋白染色体蛋白高迁移率组1（HMG-1）快速迁移到细胞核中（Rechsteiner，M.，和Kuehl，L.（1979）Cell 16，901-908）。我们分离出分泌抗HMG-1单克隆抗体的杂交瘤。这些单克隆抗体之一，FR-1，在体外抑制125 I-HMG-1的染色质从FL细胞分离的结合。当通过红细胞介导的显微注射将125 I-HMG-1与抗体FR-1共同引入时，抗体FR-1不阻止125 I-HMG-1在细胞核中的蓄积。当125 I-抗体FR-1或异硫氰酸荧光素抗体FR-1被引入FL细胞的细胞质中时，大部分抗体不积聚在细胞核中。但当125 I-或异硫氰酸荧光素抗体FR-1与HMG-1共导入FL细胞的胞质中时，它确实迁移到细胞核中。
Non-histone chromosomal protein high mobility group 1 (HMG-1) rapidly migrates into the nucleus when injected into the cytoplasm of bovine fibroblasts and HeLa cells by red cell-mediated microinjection (Rechsteiner, M., and Kuehl, L. (1979) Cell 16, 901-908). We isolated hybridomas secreting monoclonal antibodies against HMG-1. One of these monoclonal antibodies, FR-1, inhibited in vitro binding of 125I-HMG-1 to chromatin isolated from FL cells. When 125I-HMG-1 was co-introduced with antibody FR-1 by red cell-mediated microinjection, antibody FR-1 did not prevent the accumulation of 125I-HMG-1 in the nucleus. When 125I-antibody FR-1 or fluorescein isothiocyanate antibody FR-1 was introduced into the cytoplasm of FL cells, most of the antibody did not accumulate in the nucleus. But when 125I- or fluorescein isothiocyanate antibody FR-1 was co-introduced with HMG-1 into the cytoplasm of FL cells, it did migrate into the nucleus.