Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death
Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death
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DOI：
10.1007/s00299-009-0727-x
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发表时间：
2009
影响因子：
6.2
通讯作者：
C. Tateda;K. Yamashita;F. Takahashi;T. Kusano;Yoshihiro Takahashi
中科院分区：
文献类型：
--
作者：
C. Tateda;K. Yamashita;F. Takahashi;T. Kusano;Yoshihiro Takahashi
电压依赖性阴离子通道（VDAC）是线粒体外膜的主要蛋白。VDAC在哺乳动物细胞凋亡（一种程序性细胞死亡）中起着重要作用。然而，植物对应物在植物特异性细胞死亡过程中的作用，如病原菌诱导的过敏反应，却知之甚少。为了解决这个问题，我们从烟草中分离了三个VDAC全长cDNA（NtVDAC 1 -3）。推导的产物NtVDACs具有78-85%的同一性，并保留了其C-末端区域远端的保守真核线粒体孔蛋白特征。通过绿色荧光蛋白融合方法证实了三种NtVDAC在植物细胞中的线粒体定位。然后，我们讨论了有关病机关系的主要问题。那NtVDACs的本氏菌直系同源物通过用非宿主病原体菊苣假单胞菌攻击而上调，但在用毒性病原体P. syringaepv.tabaci. VDAC的药物抑制和NbVDACs基因的沉默都降低了非宿主对P的抗性。cichorii，表明VDACs参与防御非宿主病原体。使用类似的方法也验证了NbVDAC参与细胞凋亡介导的细胞死亡。
The voltage-dependent anion channel (VDAC) is a major outer mitochondrial membrane protein. It is well documented that VDAC plays an important role in apoptosis, a kind of programmed cell death, in mammalian systems. However, little is known about the role of the plant counterpart during the process of plant-specific cell death such as pathogen-induced hypersensitive response. To address this issue, we isolated threeVDACfull-length cDNAs (NtVDAC1–3) fromNicotiana tabacum. The deduced products, NtVDACs, share 78–85% identity and retain the conserved eukaryotic mitochondrial porin signature distal to their C-terminal regions. Mitochondrial localization of three NtVDACs in plant cells was confirmed via a green fluorescent protein fusion method. Then, we addressed the main issue concerning pathogenesis relation. TheN. benthamianaorthologues ofNtVDACswere upregulated by challenge with the non-host pathogenPseudomonas cichorii, but not after challenge with the virulent pathogenP. syringaepv.tabaci. Both the pharmaceutical inhibition of VDAC and silencing ofNbVDACsgenes compromised the non-host resistance againstP. cichorii, suggesting the involvement of VDACs in defense against non-host pathogen. Involvement of NbVDACs in Bax-mediated cell death was also verified using a similar approach.