Modular, cost-effective, and portable capillary gradient liquid chromatography system for on-site analysis.
Modular, cost-effective, and portable capillary gradient liquid chromatography system for on-site analysis.
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DOI：
10.1016/j.chroma.2020.461374
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Lewellwyn J. Coates;S. Lam;A. Gooley;P. Haddad;B. Paull;H. Wirth
中科院分区：
文献类型：
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作者：
Lewellwyn J. Coates;S. Lam;A. Gooley;P. Haddad;B. Paull;H. Wirth
这项工作展示了一个紧凑的，模块化的，具有成本效益的分离系统配置，以解决特定的分离问题的发展。分离原理基于梯度毛细管液相色谱法，该系统由精密步进电机驱动的便携式注射泵和可互换玻璃注射器（100 µL至1000 µL）组成。在1 µL/min至100 µL/min的典型流速范围内，实现了<1% RSD的优异流速精度，这对于毛细管柱是理想的。一个可变的外部环路体积和电动微型进样阀用于样品引入。检测基于商业Z型UV吸光度流动池，该流动池容纳在定制的冷却外壳（40 mm x 40 mm）内，该冷却外壳还包含UV-LED光源和光电二极管。使用线性梯度洗脱评价系统和色谱性能，对于使用50 mm X 530 µm ID C183 µm颗粒毛细管柱分离5组分混合物，峰面积的日间重复性RSD <1.5%（n= 6），保留时间的日间重复性RSD < 0.4%（n= 6）。该系统可以运行任何商业或内部填充柱，长度为50 mm至100 mm，ID范围为200至700 µm。开发的便携式系统使用定制的基于Windows的色谱软件进行操作，完成数据采集和系统控制。
This work demonstrates the development of a compact, modular, cost-effective separation system configured to address a specific separation problem. The principles of the separation are based on gradient capillary liquid chromatography where the system consists of precision stepper motor-driven portable syringe pumps with interchangeable glass syringes (100 µL to 1000 µL). Excellent flow-rate precision of<1% RSD was achieved with typical flow-rates ranging from 1 µL/min to 100 µL/min, which was ideal for capillary columns. A variable external loop volume and electrically actuated miniature injection valve was used for sample introduction. Detection was based upon a commercial Z-type UV absorbance flow-cell housed within a custom-built cooling enclosure (40 mm x 40 mm) which also contained a UV-LED light-source and a photodiode. System and chromatographic performance was evaluated using linear gradient elution, with day to day repeatability of <1.5% RSD (n= 6) for peak area, and < 0.4% RSD (n= 6) for retention time, for the separation of a 5 component mixture using a 50 mm X 530 µm ID C183 µm particle capillary column. The system can run any commercial or in-house packed columns from 50 mm to 100 mm length with IDs ranging from 200 to 700 µm. The developed portable system was operated using custom-built windows-based chromatography software, complete with data acquisition and system control.