Expression of the c-myb oncogene in human small cell lung carcinoma.
Expression of the c-myb oncogene in human small cell lung carcinoma.
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DOI：
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发表时间：
1985
期刊：
影响因子：
11.2
通讯作者：
C. Griffin;S. Baylin
中科院分区：
文献类型：
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作者：
C. Griffin;S. Baylin
我们发现致癌基因c-myb在人肺癌细胞系中存在差异表达，而myb同源RNA仅在小细胞肺癌（SCLC）细胞系中可以检测到。用Northern印迹法检测了13个已建立细胞系的聚腺苷酸rna与放射性标记v-myb探针的杂交能力。与v-myb同源的3.5千碱基RNA转录物存在于四种经典SCLC系中的四种和四种SCLC变异系中的三种中，但在五种非小细胞肺癌系中的五种中不存在。该转录本与未成熟髓系KG1中发现的转录本大小相同，但RNA的数量仅为KG1系的10%左右。与v-myb杂交的第二种转录物，大小为2.4千碱基，也存在于变异型SCLC系和COLO 320系中，它们都扩增了c-myc基因，并显著增加了c-myc信使RNA。myb转录本在SCLC中的存在表明，myb基因可能在一种重要的人类上皮肿瘤的发生或维持中具有特定的作用。
We have found that the oncogene c-myb is differentially expressed in human lung cancer cell lines and that myb-homologous RNA can be detected only in small cell lung cancer (SCLC) cell lines. Polyadenylic acid-RNA from 13 established cell lines was examined by Northern blotting for its ability to hybridize to a radiolabeled v-myb probe. A 3.5-kilobase RNA transcript homologous to v-myb is present in four of four lines of classic SCLC and in three of four SCLC variant lines but not in five of five non-small cell lung cancer lines tested. This transcript is the same size as that found in the immature myeloid cell lines KG1, but the amount of RNA is only about 10% of that in the KG1 line. A second transcript hybridizing to v-myb, 2.4 kilobases in size, is also present in the variant SCLC lines and the COLO 320 line, all of which have amplification of the c-myc gene and markedly increased c-myc messenger RNA. The presence of myb transcripts in SCLC suggests that the myb gene may have a specific role in the initiation or maintenance of an important human epithelial tumor.