FANCI Binds Branched DNA and Is Monoubiquitinated by UBE2T-FANCL
FANCI Binds Branched DNA and Is Monoubiquitinated by UBE2T-FANCL
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DOI：
10.1074/jbc.c109.038075
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发表时间：
2009-08-28
影响因子：
4.8
通讯作者：
Sung, Patrick
中科院分区：
文献类型：
--
作者：
Longerich, Simonne;Filippo, Joseph San;Sung, Patrick
FANCI是DNA损伤修复的Fanconi贫血（FA）途径的组成部分。在DNA损伤发生时，FANCI在Lys-523上变成单泛素化并重新定位到染色质，在染色质中它与单泛素化的FANCD 2一起发挥作用以促进DNA修复。我们表明，FANCI和它的C-末端片段具有DNA结合活性，喜欢分支结构。我们还证明了FANCI可以在体外通过UBE 2 T-FANCL对在Lys-523上泛素化。这些研究结果应有助于未来的努力，旨在阐明分子方面的FA途径。
FANCI is integral to the Fanconi anemia (FA) pathway of DNA damage repair. Upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 and relocalizes to chromatin, where it functions with monoubiquitinated FANCD2 to facilitate DNA repair. We show that FANCI and its C-terminal fragment possess a DNA binding activity that prefers branched structures. We also demonstrate that FANCI can be ubiquitinated on Lys-523 by the UBE2T-FANCL pair in vitro. These findings should facilitate future efforts directed at elucidating molecular aspects of the FA pathway.