First Record of a New Member of Anopheles Hyrcanus Group From Iran: Molecular Identification, Diagnosis, Phylogeny, Status of kdr Resistance and Plasmodium Infection
First Record of a New Member of Anopheles Hyrcanus Group From Iran: Molecular Identification, Diagnosis, Phylogeny, Status of kdr Resistance and Plasmodium Infection
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DOI：
10.1603/033.046.0515
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Zakeri, S.
中科院分区：
文献类型：
--
作者：
Djadid, N. Dinparast;Jazayeri, H.;Zakeri, S.
目前的研究旨在提供进一步的证据，在Ardebil，Giulan，和胡泽斯坦省的物种组成，杀虫剂抗性，和媒介能力的成员内的Anopheles（Anopheles）Hyreanus组。通过对核糖体DNA内转录间隔区2（ITS 2）基因序列的测定，鉴定了赫坎按蚊复合体的两个成员：赫坎按蚊Pallas和一个新的世界纪录种/型，即赫坎按蚊（Anopheles hyrcanus sp，IR）。此外，我们确定和比较的部分外显子I和II和整个内含子I区的杀虫剂抗性相关的电压门控钠通道（vgse）基因在赫坎组和其他主要东半球按蚊种群。利用Hyreanus组成员和其他按蚊属种的ITS 2和vgsc序列构建系统发育树，揭示了Hyrcanus组内西部和东部古北区类群之间的进化关系。巢式聚合酶链反应检测疟原虫种揭示了恶性疟原虫感染，在一个。hyrcanus采自Guilan省Fooman地区。这项研究的数据导致了Hyrcanus组内的新成员/形式的引入，在其vgsc基因中鉴定和定义了与拟除虫菊酯和滴滴涕相关的敲低抗性状态，检测了疟原虫感染，并进一步证明了这些分类群内的遗传相关性。总体结果可能建议重新考虑An的作用。hyrcanus在疟疾传播中的作用，为古北西部地区疟疾防治项目的实施和评价提供了依据。
The current study aimed to provide further evidence on the status of species composition, insecticide resistance, and vectorial capacity within the members of Anopheles (Anopheles) Hyreanus Group in Ardebil, Giulan, and Khuzestan provinces of Iran. Sequencing the internal transcribed spacer 2 (ITS2) of ribosomal DNA gene led to identification of two members of Hyrcanus complex: Anopheles hyrcanus Pallas and a new species/form, hereafter called Anopheles hyrcanus sp(IR), as a world record. Furthermore, we identified and compared partial sequences of exons I and II and the whole intron I region of insecticide resistance-related voltage-gated sodium channel (vgse) gene in populations of Hyrcanus Group and other main old world Anopheles species. The ITS2 and vgsc sequences in members of Hyreanus Group and other Anopheles species were used for construction of phylogenetic tree, which demonstrated the evolutionary relatedness among Western and Eastern Palearctic taxa within the Hyrcanus Group. A nested polymerase chain reaction assay for detection of Plasmodium species revealed the infection of Plasmodium falciparum, within An. hyrcanus collected from Fooman district in Guilan province. The data from this study led to the introduction of a new member/form within the Hyrcanus Group, identification and definition of the status of knockdown resistance related to pyrethroids and DDT in their vgsc gene, detection of Plasmodium infection, and further evidence on genetic relatedness within these taxa. The overall results may suggest reconsidering the role of An. hyrcanus in malaria transmission, which would be useful for implementation and evaluation of malaria control programs in Western Palearctic region.