In vitro fermentation of flaxseed polysaccharide by fecal bacteria inhibits energy intake and adipogenesis at physiological concentration
In vitro fermentation of flaxseed polysaccharide by fecal bacteria inhibits energy intake and adipogenesis at physiological concentration
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DOI：
10.1016/j.foodres.2020.109920
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发表时间：
2021-01-01
影响因子：
8.1
通讯作者：
Peng, Xichun
中科院分区：
文献类型：
--
作者：
Lin, Xiaohong;Xu, Weiye;Peng, Xichun
肥胖及其相关的代谢紊乱已成为全球性的流行病。最近，我们发现亚麻籽多糖（FP）的抗肥胖作用可以通过调节肠道微生物群来实现。FP的减肥作用主要是通过与FP相互作用产生的代谢产物来实现的，这一点还有待进一步阐明。本研究探讨了粪菌发酵FP代谢产物对体外能量代谢和脂肪生成的影响。通过肠葡萄糖转运蛋白（包括钠依赖性葡萄糖转运蛋白1（SGLT 1）和葡萄糖转运蛋白2（GLUT 2））的mRNA和蛋白表达以及肠Caco-2细胞的葡萄糖摄取来分析能量代谢的影响。通过油红O染色观察3 T3-L1细胞内脂滴的变化，并检测3 T3-L1细胞内过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor，PPAR）γ、CCAAT增强子结合蛋白（CCAAT enhancer-binding proteins，C/EBP）α和β的mRNA和蛋白表达。结果表明，该代谢产物通过下调Caco-2细胞GLUT 2和SGLT 1的mRNA和蛋白表达，显著抑制葡萄糖摄入。此外，它们还通过下调分化中的脂肪细胞中的PPAR γ、C/EBP α和C/EBP β的mRNA和蛋白表达而导致脂质积聚的减少。对能量摄入和脂肪生成的抑制作用具有浓度依赖性，其中生理浓度的代谢产物的抑制作用最为显著。在生理浓度下，粪菌代谢产物对能量摄入和脂肪生成有抑制作用，这可能是FP日粮的减肥机制之一。
Obesity and its related metabolic disorders have been a global pandemic. Recently, we found an anti-obesity effect of flaxseed polysaccharide (FP) that could be achieved by regulating intestinal microbiota. The antiobesity effect of FP is mainly attributed to the metabolites produced by the interaction with FP, which remains to be elucidated. In this research, the in vitro effects of metabolites of FP fermented by fecal bacteria on energy metabolism and adipogenesis were investigated. The effect of energy metabolism was analyzed by mRNA and protein expression of the intestinal glucose transporters, including sodium dependent glucose transporter (SGLT1) and glucose transporter 2 (GLUT2), and glucose uptake in intestinal Caco-2 cells. The lipogenic effect were evaluated by Oil red O staining of intracellular lipid droplets and the mRNA and protein expression of peroxisome proliferator-activated receptor (PPAR) gamma, CCAAT-enhancer-binding proteins (C/EBP) alpha and beta in 3T3-L1 cells. The results showed the metabolites significantly inhibited glucose intake through downregulating the mRNA and protein expression of GLUT2 and SGLT1 in Caco-2 cells. Besides, they also led to the decrease of lipid accumulation through downregulating the mRNA and protein expression of PPAR gamma, C/EBP alpha, and C/EBP beta in differentiating adipocytes. The inhibitory effects on energy intake and adipogenesis were concentration dependent, and metabolites at physiological concentration showed the most significant effect. Metabolites of fecal bacteria fermenting FP inhibited energy intake and adipogenesis at physiological concentration, which might be one of the weight-loss mechanisms of FP-diet.