Visualizing phosphatidylcholine via mass spectrometry imaging: relevance to human health
Visualizing phosphatidylcholine via mass spectrometry imaging: relevance to human health
复制标题
DOI：
10.1080/14789450.2018.1526679
复制
发表时间：
2018-09
影响因子：
3.4
通讯作者：
Jenny Leopold;Yulia Popkova;K. Engel;J. Schiller
中科院分区：
文献类型：
--
作者：
Jenny Leopold;Yulia Popkova;K. Engel;J. Schiller
摘要简介：质谱成像（MSI）技术如今被广泛用于从生物组织获取空间分辨的代谢信息。由于（磷酸）脂质存在于所有动物组织中并且可以非常灵敏地检测，因此它们通常是此类研究的焦点。这尤其适用于磷脂酰胆碱 (PC)，由于其胆碱头基具有永久正电荷，因此可以非常灵敏地检测为正离子。涵盖的领域：在简短介绍生物系统中存在的脂质种类和通常用于获得空间分辨质谱的方法（重点是基质辅助激光解吸/电离耦合到飞行时间（MALDI-TOF）MSI）后，将调查迄今为止以PC成分变化为特征的疾病。专家评论：由于 MS 可以非常灵敏地检测 PC 物种，因此灵敏度不是主要问题。然而，空间分辨率仍然有限，并且 MALDI-TOF MSI 很难解析细胞尺寸，这是可用方法的关键点。由于缺乏可重复性和标准化，需要进一步开发。
ABSTRACT Introduction: Mass spectrometry imaging (MSI) techniques are nowadays widely used to obtain spatially resolved metabolite information from biological tissues. Since (phospho)lipids occur in all animal tissues and are very sensitively detectable, they are often in the focus of such studies. This particularly applies for phosphatidylcholines (PC) which are very sensitively detectable as positive ions due to the permanent positive charge of their choline headgroup. Areas covered: After a short introduction of lipid species occurring in biological systems and approaches normally used to obtain spatially resolved mass spectra (with the focus on matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) MSI) a survey will be given which diseases have so far been characterized by changes of the PC composition. Expert commentary: Since PC species are very sensitively detectable by MS, sensitivity is not a major issue. However, spatial resolution is still limited and cellular dimensions can be hardly resolved by MALDI-TOF MSI, which is a critical point of the available approaches. Due to lacks of reproducibility and standardization further development is required.