Expression of PEBP4 protein correlates with the invasion and metastasis of colorectal cancer
Expression of PEBP4 protein correlates with the invasion and metastasis of colorectal cancer
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DOI：
10.1007/s13277-011-0279-x
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发表时间：
2012-02-01
期刊：
影响因子：
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通讯作者：
Jia, Baoqing
中科院分区：
文献类型：
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作者：
Liu, Hongyi;Kong, Qingling;Jia, Baoqing
本研究旨在探讨PEBP 4蛋白在大肠癌组织中的表达及其与大肠癌临床病理的相关性，探讨PEBP 4表达与大肠癌细胞侵袭转移的关系，为大肠癌的生物学治疗提供实验依据。采用RT-PCR和western blot方法分别检测大肠癌组织和癌旁正常组织中PEBP 4 mRNA和蛋白的表达，分析其与大肠癌发生发展及临床病理的相关性。利用RNA干扰技术，敲低PEBP 4在人大肠癌细胞HCT 116中的表达，观察其侵袭能力的变化。PEBP 4在大肠癌组织中的阳性表达率显著高于癌旁正常组织（p < 0.05）。在有淋巴结转移和远处转移的癌组织中，阳性表达率显著高于无淋巴结转移和远处转移的癌组织（p < 0.05）。PEBP 4在早期（I、II）患者癌组织中的阳性表达率显著低于晚期（III、IV）患者（p < 0.05）。大肠癌组织分化程度越低，PEBP 4 mRNA阳性表达率越高（p < 0.05）。然而，这与患者的性别、年龄和肿瘤大小无关（p > 0.05）。在结直肠癌组织中，PEBP 4蛋白的表达与其mRNA的表达一致。即PEBP 4蛋白在大肠癌组织中的表达显著高于癌旁正常组织在有淋巴结转移和远处转移的患者中，其表达明显高于无淋巴结转移的患者（P < 0.05）（p < 0.05），并且结肠直肠癌中较低的分化程度对应于较高的TNM分期沿着较高的PEBP 4蛋白表达（p < 0.05）。在用PEBP 4 siRNA转染HCT 116细胞后，它们显示出显著较低的PEBP 4蛋白表达水平（p < 0.05），并且与未转染或转染对照相比，通过Transwell室的细胞数量显著较低（p < 0.05）。PEBP 4蛋白的过度表达可能与大肠癌的发生、发展、转移和侵袭有关。
This study aimed to investigate the expression of PEBP4 protein in colorectal carcinoma tissues and its correlation with the clinical pathology of colorectal cancer and to investigate the relationship between PEBP4 expression and the invasion and metastasis of colorectal cancer cells, which could provide an experimental basis for future biological treatments of human colorectal cancer. RT-PCR and western blot methods were applied to detect the mRNA and protein expressions, respectively, of PEBP4 in colorectal cancer tissues and normal pericarcinoma tissues, and their correlations with the tumorigenesis and development of colorectal cancer, as well as its clinical pathology, were analyzed. Using the RNA interference technology, the expression of PEBP4 was knocked down in the human colorectal cancer cell HCT116, and the changes of the invasion capability of HCT116 were monitored. The positive mRNA expression rate of PEBP4 in colorectal cancer tissue was significantly higher than that in the normal pericarcinoma tissue (p < 0.05). Also, the positive expression rate in the cancer tissues from patients with positive lymph node and distant metastasis was significantly higher than that from the patients negative for lymph node and distant metastasis (p < 0.05). The positive expression rate of PEBP4 in the cancer tissues from the patients in early stages (I, II) was significantly lower than the expression rate in patients in advanced stages (III, IV) (p < 0.05). A lower degree of differentiation in colorectal cancer corresponded to a higher positive mRNA expression rate of PEBP4 (p < 0.05). However, this was independent of the patient's gender, age, and tumor size (p > 0.05). In colorectal cancer tissue, the expression of PEBP4 protein was consistent with its mRNA. Namely, PEBP4 protein expression in colorectal cancer tissues was significantly higher than that in the normal pericarcinoma tissues (p < 0.05), the expression in the cancer tissues from the patients with positive lymph node and distant metastasis was significantly higher than that from the patients who were negative for these metastases (p < 0.05), and a lower degree of differentiation in colorectal cancer corresponded to a higher TNM staging along with a higher PEBP4 protein expression (p < 0.05). After HCT116 cells transfected with PEBP4 siRNA, they showed a significantly lower expression level of PEBP4 protein (p < 0.05), and the number of cells that passed through the Transwell chamber was significantly lower compared to the non-transfected or the transfected controls (p < 0.05). The over-expression of PEBP4 protein may be related to the tumorigenesis, development, metastasis, and invasion of colorectal cancer.