The bacteriophage T4 regA gene: primary sequence of a translational repressor.
The bacteriophage T4 regA gene: primary sequence of a translational repressor.
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噬菌体 T4 regA 基因：翻译抑制子的一级序列。
DOI：
10.1093/nar/12.15.5979
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发表时间：
1984
影响因子：
14.9
通讯作者：
Gold,L
中科院分区：
文献类型：
--
作者：
Trojanowska,M;Miller,ES;Karam,J;Stormo,G;Gold,L
噬菌体T4的regA基因产物是一种自身控制的翻译调节蛋白，在T4编码的“早期”mRNA种类的亚群的差异抑制（翻译抑制）中起作用。该多肽的结构基因定位在噬菌体DNA复制基因簇（基因45-44-62-regA-43-42）内，除了其中一个（基因43）之外，所有基因都处于regA介导的翻译控制下。我们已经克隆了T4 regA基因，确定其核苷酸序列，并确定了质粒编码的hyperproducedregA蛋白的氨基末端残基。结果表明，T4 regA基因产物是一个122个氨基酸的多肽，是温和的碱性和亲水性的字符，这些功能是与已知的特性来自T4感染的细胞的蛋白质是一致的。对regA基因及其三个上游邻居（基因45、44和62）的核苷酸序列的计算机辅助分析表明，在这个四基因簇中存在三个翻译起始单位;一个为基因45，一个为基因44、62和regA，一个仅为regA基因服务。分析还表明，基因447-62翻译单元具有稳定的RNA结构，该结构使这两个基因的翻译偶联成为必需。
TheregA gene product of bacteriophage T4 is an autogenously controlled translational regulatory protein that plays a role In differential inhibition (translational repression) of a subpopulation of T4-encoded “early” mRNA species. The structural gene for this polypeptide maps within a cluster of phage DNA replication genes, (genes 45-44-62-regA-43-42), all but one of which (gene 43) are underregA-mediated translational control. We have cloned the T4regA gene, determined its nucleotide sequence, and identified the amino-terminal residues of a plasmid-encoded, hyperproducedregA protein. The results suggest that the T4regA gene product is a 122 amino acid polypeptide that is mildly basic and hydrophilic in character; these features are consistent with known properties of protein derived from T4-infected cells. Computer-assisted analyses of the nucleotide sequences of theregA gene and its three upstream neighbors (genes 45, 44, and 62) suggest the existence of three translational initiation units in this four-gene cluster; one for gene 45, one for genes 44, 62 andregA, and one that serves only theregA gene. The analyses also suggest that the gene 447–62 translational unit harbors a stable RNA structure that obligates translational coupling of these two genes.