Diesel exhaust particle-exposed human bronchial epithelial cells induce dendritic cell maturation and polarization via thymic stromal lymphopoietin
Diesel exhaust particle-exposed human bronchial epithelial cells induce dendritic cell maturation and polarization via thymic stromal lymphopoietin
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DOI：
10.1007/s10875-007-9149-0
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发表时间：
2008-03-01
影响因子：
9.1
通讯作者：
Reibman, Joan
中科院分区：
文献类型：
--
作者：
Bleck, Bertram;Tse, Doris B.;Reibman, Joan
人类暴露于空气污染物，包括环境颗粒物，已被认为是过敏性疾病增加的一种机制。柴油机排气颗粒物是环境颗粒物的主要成分，可诱导对新过敏原的致敏，但致敏发生的机制尚不清楚。我们发现，柴油机废气颗粒上调胸腺基质淋巴细胞生成素在人支气管上皮细胞中的氧化剂依赖性的方式。柴油机尾气颗粒物诱导的胸腺基质淋巴细胞生成素与髓样树突状细胞的成熟有关，这可被抗胸腺基质淋巴细胞生成素抗体或沉默上皮细胞来源的胸腺基质淋巴细胞生成素阻断。树突状细胞暴露于柴油机尾气颗粒处理的人支气管上皮细胞诱导Th2极化的胸腺基质淋巴细胞生成素依赖的方式。这些发现为柴油机尾气颗粒物改变人肺粘膜免疫的机制提供了新的见解。
Human exposure to air pollutants, including ambient particulate matter, has been proposed as a mechanism for the rise in allergic disorders. Diesel exhaust particles, a major component of ambient particulate matter, induce sensitization to neoallergens, but the mechanisms by which sensitization occur remain unclear. We show that diesel exhaust particles upregulate thymic stromal lymphopoietin in human bronchial epithelial cells in an oxidant-dependent manner. Thymic stromal lymphopoietin induced by diesel exhaust particles was associated with maturation of myeloid dendritic cells, which was blocked by anti-thymic stromal lymphopoietin antibodies or silencing epithelial cell-derived thymic stromal lymphopoietin. Dendritic cells exposed to diesel exhaust particle-treated human bronchial epithelial cells induced Th2 polarization in a thymic stromal lymphopoietin-dependent manner. These findings provide new insight into the mechanisms by which diesel exhaust particles modify human lung mucosal immunity.