TEMPORALLY SPECIFIC INVOLVEMENT OF CELL-SURFACE BETA-1,4 GALACTOSYLTRANSFERASE DURING MOUSE EMBRYO MORULA COMPACTION
TEMPORALLY SPECIFIC INVOLVEMENT OF CELL-SURFACE BETA-1,4 GALACTOSYLTRANSFERASE DURING MOUSE EMBRYO MORULA COMPACTION
复制标题
DOI：
10.1016/0092-8674(88)90496-5
复制
发表时间：
1988-04-08
期刊：
影响因子：
64.5
通讯作者：
SHUR, BD
中科院分区：
文献类型：
--
作者：
BAYNA, EM;SHAPER, JH;SHUR, BD
细胞表面β-1，4半乳糖基转移酶(GalTase)显示介导胚胎癌细胞(EC)细胞之间的细胞间粘附，特别是在植入前小鼠胚胎的晚期桑葚胚压缩过程中。针对亲和纯化的牛β-1,4 GalTase产生的单特异性抗GalTase IgG识别F9 EC细胞GalTase，如通过免疫沉淀和GalTase活性的抑制以及通过单个52kd代谢标记的膜蛋白的免疫沉淀来判断的。抗 GalTase IgG 抑制 EC 细胞之间的细胞粘附，解离致密的小鼠桑葚胚，并抑制囊胚形成。抗 GalTase IgG 特异性抑制桑葚胚压实后期的细胞粘附，这与通过直接酶测定测定的表面 GalTase 活性峰值一致。在 EC 细胞上，GalTase 活性可以从完整细胞中蛋白水解释放，并通过间接免疫荧光定位到细胞间接触区域，这与其在细胞粘附中的作用一致。 β-1,4 GalTase是第一个被鉴定的细胞粘附分子，其参与桑葚胚压实后期（继uvomorulin功能之后）。
Cell surface .beta.-1,4 galactosyltransferase (GalTase) is shown to mediate intercellular adhesions between embryonal carcinoma (EC) cells and specifically during late morula compaction in the preimplantation mouse embryo. Monospecific anti-GalTase IgG raised against affinity-purified bovine .beta.-1,4 GalTase recognizes F9 EC cell GalTase as judged by immunoprecipitation and inhibition of GalTase activity, as well as by immunoprecipitation of a single 52 kd metabolically labeled membrane protein. Anti-GalTase IgG inhibits cell adhesions between EC cells, dissociates compacted mouse morulae, and inhibits blastocyst formation. Anti-GalTase IgG specifically inhibits cell adhesions during late morula compaction, coincident with a peak of surface GalTase activity as determined by direct enzyme assay. On EC cells, GalTase activity can be proteolytically released from intact cells, and is localized by indirect immunofluorescence to areas of intercellular contact, consistent with its proposed role in cell adhesion. .beta.-1,4 GalTase is the first cell adhesion molecule identified that participates during late morula compaction, subsequent to uvomorulin function.