Genomic Diversity of Pigeon Pea (Cajanus cajan L. Millsp.) Endosymbionts in India and Selection of Potential Strains for Use as Agricultural Inoculants.
Genomic Diversity of Pigeon Pea (Cajanus cajan L. Millsp.) Endosymbionts in India and Selection of Potential Strains for Use as Agricultural Inoculants.
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DOI：
10.3389/fpls.2021.680981
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发表时间：
2021
影响因子：
5.6
通讯作者：
Poole PS
中科院分区：
文献类型：
--
作者：
Jorrin B;Maluk M;Atoliya N;Kumar SC;Chalasani D;Tkacz A;Singh P;Basu A;Pullabhotla SV;Kumar M;Mohanty SR;East AK;Ramachandran VK;James EK;Podile AR;Saxena AK;Rao D;Poole PS
木豆 (Cajanus cajan L. Millsp.) 是一种豆科作物，因其耐干旱而能适应气候变化。它由亚洲和非洲半干旱和热带次区域数百万资源匮乏的农民种植，是其营养粮食安全的主要贡献者。木豆是世界第六重要的豆类植物，印度占总产量的 70% 以上，并拥有多种品种。但旱地条件下木豆产量低、产量不稳定等问题亟待改善。这可以通过增强作物结瘤来实现，从而通过应用“精英”接种剂提供有效的共生根瘤菌来增强生物固氮（BNF）。因此，本研究的主要目的是分离和基因组分析可能适应干旱条件的有效根瘤菌菌株。因此，木豆内共生体是从印度南部、中部和北部的不同土壤类型中分离出来的。在对分离菌株的结瘤和促进木豆生长的能力进行功能表征后，选择了 19 个菌株进行全基因组测序，以及从 ICRISAT 培养物保藏中心获得的 8 个商业接种菌株。系统发育分析[平均核苷酸同一性MUMmer (ANIm)]显示木豆内共生体是慢生根瘤菌属和Ensifer属的成员。基于 nodC 系统发育和 nod 簇同线性，圆明慢生根瘤菌被认为是最常见的内共生体，其 nod 基因与卡佳尼慢生根瘤菌和湛江慢生根瘤菌相似。这种共生体类型（例如，来自中央邦的菌株 BRP05）也优于在木豆上测试的所有其他菌株，但来自印度北部的 Ensifer 碱化菌株（NBAIM29）是一个明显的例外。研究结果为木豆接种剂的开发提供了基础，通过使用针对印度不同农业气候地区的有效固氮根瘤菌来提高木豆的产量。
Pigeon pea (Cajanus cajan L. Millsp. ) is a legume crop resilient to climate change due to its tolerance to drought. It is grown by millions of resource-poor farmers in semiarid and tropical subregions of Asia and Africa and is a major contributor to their nutritional food security. Pigeon pea is the sixth most important legume in the world, with India contributing more than 70% of the total production and harbouring a wide variety of cultivars. Nevertheless, the low yield of pigeon pea grown under dry land conditions and its yield instability need to be improved. This may be done by enhancing crop nodulation and, hence, biological nitrogen fixation (BNF) by supplying effective symbiotic rhizobia through the application of “elite” inoculants. Therefore, the main aim in this study was the isolation and genomic analysis of effective rhizobial strains potentially adapted to drought conditions. Accordingly, pigeon pea endosymbionts were isolated from different soil types in Southern, Central, and Northern India. After functional characterisation of the isolated strains in terms of their ability to nodulate and promote the growth of pigeon pea, 19 were selected for full genome sequencing, along with eight commercial inoculant strains obtained from the ICRISAT culture collection. The phylogenomic analysis [Average nucleotide identity MUMmer (ANIm)] revealed that the pigeon pea endosymbionts were members of the genera Bradyrhizobium and Ensifer. Based on nodC phylogeny and nod cluster synteny, Bradyrhizobium yuanmingense was revealed as the most common endosymbiont, harbouring nod genes similar to those of Bradyrhizobium cajani and Bradyrhizobium zhanjiangense. This symbiont type (e.g., strain BRP05 from Madhya Pradesh) also outperformed all other strains tested on pigeon pea, with the notable exception of an Ensifer alkalisoli strain from North India (NBAIM29). The results provide the basis for the development of pigeon pea inoculants to increase the yield of this legume through the use of effective nitrogen-fixing rhizobia, tailored for the different agroclimatic regions of India.
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影响因子：
3.5
作者：
Kaneko T;Maita H;Hirakawa H;Uchiike N;Minamisawa K;Watanabe A;Sato S
通讯作者：
Sato S
影响因子：
2.6
作者：
Kim, Won-Seok;Krishnan, Hari B.
通讯作者：
Krishnan, Hari B.
DOI：
10.1073/pnas.0409727102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Konstantinidis, KT;Tiedje, JM
通讯作者：
Tiedje, JM
影响因子：
4.4
作者：
Agrawal S;Arze C;Adkins RS;Crabtree J;Riley D;Vangala M;Galens K;Fraser CM;Tettelin H;White O;Angiuoli SV;Mahurkar A;Fricke WF
通讯作者：
Fricke WF
CLOVR：使用云计算从桌面上自动化和便携式序列分析的虚拟机。
影响因子：
3
作者：
Angiuoli SV;Matalka M;Gussman A;Galens K;Vangala M;Riley DR;Arze C;White JR;White O;Fricke WF
通讯作者：
Fricke WF