EFFECTS OF PURIFIED HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR AND OTHER SUBSTANCES ON SECRETION OF ADRENOCORTICOTROPIN AND BETA-ENDORPHIN-LIKE IMMUNOACTIVITIES INVITRO
EFFECTS OF PURIFIED HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR AND OTHER SUBSTANCES ON SECRETION OF ADRENOCORTICOTROPIN AND BETA-ENDORPHIN-LIKE IMMUNOACTIVITIES INVITRO
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DOI：
10.1210/endo-103-5-1910
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发表时间：
1978-01-01
期刊：
影响因子：
4.8
通讯作者：
GUILLEMIN, R
中科院分区：
文献类型：
--
作者：
VALE, W;RIVIER, C;GUILLEMIN, R
各种物质对- β分泌的影响。研究了[大鼠]垂体前细胞原代培养-内啡肽样免疫活性（. β .- end -li）或acth样免疫活性（. β .- end -li）。培养中等水平的β。-END-LI和ACTH-LI通过RIA[放射免疫测定]针对。β细胞的Leu14-His27区域进行测定。-内啡肽和ACTH的n端区域。一般的促分泌剂，如8Br-AMP、3-异丁基甲基黄嘌呤和升高的培养基[K+]，刺激这两种β的分泌。-END-LI和ACTH-LI。更具体的促肾上腺皮质激素分泌剂，如去甲肾上腺素、抗利尿激素和一种高度纯化的绵羊下丘脑促肾上腺皮质激素释放因子（hypcrf）制剂，可增加. β的分泌率。-END-LI和ACTH-LI。糖皮质激素、地塞米松-21- po4、黄体酮；和前列腺素（PGE2）抑制crf介导的。-END-LI和ACTH-LI。有多个峰值。-END-LI和ACTH-LI在变性条件下通过biogelp -60培养基的凝胶过滤分离。在未受刺激细胞的培养基中。观察到-END-LI峰，用31K ACTH， . β洗脱。-脂促素和。脑内啡(度量。-LPH61-91)，而ACTH的峰值分别出现在ACTH为31K时和ACTH为ACTH时。在hp - crf或8Br-AMP刺激下，培养细胞的培养基具有更大的免疫反应峰。脂解激素,度量。-内啡肽和ACTH。来自受刺激细胞的液体含有一个额外的ACTH-LI峰，它被洗脱为大约6.5道尔顿的部分。纯化的hmp - crf和其他可能的调节剂对β的分泌有类似的作用。-END-LI和ACTH-LI。
The effects of various substances on the secretion of .beta.-endorphin-like immunoactivities (.beta.-END-LI) or ACTH-like immunoactivities (ACTH-LI) by primary [rat] anterior pituitary cell cultures were studied. Culture medium levels of .beta.-END-LI and ACTH-LI were estimated by RIA [radioimmunoassays] directed against the Leu14-His27 region of .beta.-endorphin and the N-terminal region of ACTH. General secretagogues, such as 8Br-AMP, 3-isobutyl methyl xanthine and elevated medium [K+], stimulate the secretion of both .beta.-END-LI and ACTH-LI. More specific ACTH secretagogues, such as norepinephrine, vasopressin and a highly purified preparation of ovine hypothalamic corticotropin-releasing factor (HYP-CRF), increase the secretory rates of .beta.-END-LI as well as ACTH-LI. The glucocorticoid, dexamethasone-21-PO4, progesterone; and prostaglandin, PGE2, inhibit the CRF-mediated secretion of .beta.-END-LI and ACTH-LI. Multiple peaks of .beta.-END-LI and ACTH-LI were resolved by gel filtration of culture fluids on Biogel P-60 under denaturing conditions. In media from nonstimulated cells, .beta.-END-LI peaks were observed which coeluted with 31K ACTH, .beta.-lipotropin and .beta.-endorphin (.beta.-LPH61-91), while peaks of ACTH were seen which coeluted with 31K ACTH and with ACTH. Media from cultured cells stimulated by HYP-CRF or 8Br-AMP had much larger peaks of immunoreactivities coeluting with .beta.-lipotropin, .beta.-endorphin and ACTH. Fluids from stimulated cells contained an additional peak of ACTH-LI, which eluted as an approximate 6.5 dalton moiety. Purified HYP-CRF and other putative regulatory agents have similar effects on the secretions of .beta.-END-LI and ACTH-LI in vitro.