THE ABILITY OF SYNOVIOCYTES TO SUPPORT TERMINAL DIFFERENTIATION OF ACTIVATED B-CELLS MAY EXPLAIN PLASMA-CELL ACCUMULATION IN RHEUMATOID SYNOVIUM
THE ABILITY OF SYNOVIOCYTES TO SUPPORT TERMINAL DIFFERENTIATION OF ACTIVATED B-CELLS MAY EXPLAIN PLASMA-CELL ACCUMULATION IN RHEUMATOID SYNOVIUM
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DOI：
10.1172/jci117685
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发表时间：
1995-02-01
影响因子：
15.9
通讯作者：
MIOSSEC, P
中科院分区：
文献类型：
--
作者：
DECHANET, J;MERVILLE, P;MIOSSEC, P
为了了解RA滑膜内浆细胞的积累，研究了类风湿性滑膜细胞支持B细胞分化为浆细胞的能力。扁桃体B淋巴细胞在融合单层滑膜细胞上培养，分泌的Ig（主要是IgM）比直接在塑料孔上培养的B细胞多3倍。更重要的是，滑膜细胞提高了14倍的生产免疫球蛋白（主要是IgG）的B细胞共刺激金黄色葡萄球菌科万（SAC）颗粒。IL-10和IL-2在较低程度上增加共培养物中的IG分泌，并且它们的组合是协同的。在SAC、IL-2和IL-10的存在下，滑膜细胞增加13-884倍的Igc产生，其达到0.19 ng/细胞/天。RA以及正常滑膜细胞比其他贴壁细胞系更有效地支持终末B细胞分化。滑膜细胞活性既支持B细胞存活，又诱导B细胞终末分化为具有典型形态、高水平胞浆内Ig和CD 20（-）CD 38（高）表面表达的成熟浆细胞。目前的观察应允许识别参与B细胞成熟为浆细胞，并在类风湿性滑膜中积累的分子。
To understand the accumulation of plasma cells within RA synovium, the ability of rheumatoid synoviocytes to support the differentiation of B cells into plasma cells was explored. Tonsillar B lymphocytes cultured over confluent monolayers of synoviocytes, secreted threefold more Igs (mainly IgM) than B cells cultured directly on plastic well. More importantly, synoviocytes enhanced by 14-fold the production of Igs (mainly IgG) by B cells costimulated with Staphylococcus aureus Cowan (SAC) particles. IL-10 and, in a lower extent, IL-2 increased Ig secretion in cocultures, and their combination was synergistic. In the presence of SAC, IL-2, and IL-10, synoviocytes increased by 13-884-fold the production of Igc, which reached 0.19 ng/cell per day. RA as well as normal synoviocytes were more potent than other adherent cell lines to support terminal B cell differentiation. Synoviocyte activity involved both a support of B cell survival, and an induction of the terminal differentiation of B cells into mature plasma cells with typical morphology, high levels of intracytoplasmic Igs, and CD20(-) CD38(high) surface expression. The present observation should permit the identification of molecules involved in the maturation of B cells into plasma cells, and in their accumulation in rheumatoid synovium.