EFFECT OF COLLICULAR PROTEOGLYCAN ON THE SURVIVAL OF ADULT-RAT RETINAL GANGLION-CELLS FOLLOWING AXOTOMY
EFFECT OF COLLICULAR PROTEOGLYCAN ON THE SURVIVAL OF ADULT-RAT RETINAL GANGLION-CELLS FOLLOWING AXOTOMY
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DOI：
10.1111/j.1460-9568.1995.tb01024.x
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发表时间：
1995-01-01
影响因子：
3.4
通讯作者：
BENNETT, MR
中科院分区：
文献类型：
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作者：
HUXLIN, KR;DREHER, B;BENNETT, MR
与许多以前的研究一致，我们发现，在成年大鼠中，通过注射到视网膜受体核中进行逆行预标记的细胞在视神经眶内切片后1周丢失了29%，这个数字在轴突切断后2周增加到76%。眼内注射150 ng的480 kDa的硫酸软骨素蛋白聚糖纯化的新生大鼠的上级丘后，每三天轴突切断。这种方法导致视神经切断后7天和14天分别只有3%和28%的轴突切断的视网膜神经节细胞丢失。眼内注射C型硫酸软骨素（存在于丘蛋白聚糖上的糖类型之一）也导致轴突切断的神经节细胞的显著保存（在视神经损伤后14天仅损失48%）。这些研究结果表明，丘蛋白聚糖，并在较小程度上其糖部分，大大减缓成年视网膜神经节细胞轴突切断术后的变性。
Consistent with numerous previous studies, we have found that in adult rats 29% of cells retrogradely prelabelled by injections into retino-recipient nuclei are lost 1 week after intraorbital section of the optic nerve, This figure increases to 76% 2 weeks after axotomy. Intraocular injections of 150 ng of 480 kDa chondroitin sulphate proteoglycan purified from the superior colliculi of neonatal rats were performed every third day after axotomy. This procedure resulted in the loss of only 3 and 28% of the axotomized retinal ganglion cells 7 and 14 days respectively after optic nerve section. Intraocular injections of chondroitin sulphate type C, one of the sugar types present on the collicular proteoglycan, also resulted in a significant saving of axotomized ganglion cells (with the loss of only 48% 14 days after optic nerve lesion). These findings suggest that the collicular proteoglycan, and to a lesser extent its sugar moieties, substantially slows down the degeneration of adult retinal ganglion cells following axotomy.