SEQUENCE VARIATION OF HUMAN PAPILLOMAVIRUS TYPE-16 E7 IN PREINVASIVE AND INVASIVE CERVICAL NEOPLASIAS
SEQUENCE VARIATION OF HUMAN PAPILLOMAVIRUS TYPE-16 E7 IN PREINVASIVE AND INVASIVE CERVICAL NEOPLASIAS
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DOI：
10.1007/bf01703438
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发表时间：
1994-09-01
期刊：
影响因子：
1.6
通讯作者：
FUJINAGA, K
中科院分区：
文献类型：
--
作者：
FUJINAGA, Y;OKAZAWA, K;FUJINAGA, K
分析宫颈上皮内瘤变(CIN)前病变组织和宫颈浸润性癌组织中HPV16E7基因核苷酸序列的变异情况。对扩增产物进行直接DNA测序的不同框架用于5‘端标记的扩增产物，即使在HPV拷贝数相对较低的标本中也能产生背景较低的明显的序列梯形。在14例宫颈肿瘤中，II例表现出与HPV16原型的序列多样性，共检测到22个核苷酸交换。其中9例导致单一氨基酸交换：1例为[THR]至[Lys(5)]，8例为[Asn(29)]至[Ser(29)]。[Ser(29)]E7在浸润性癌和癌前病变军团中均匀分布，在正常宫颈中也有表达。[Lys(5)]E7和[Ser(29)]E7在大鼠胚胎成纤维细胞中具有与原型E7相似的转化潜能，通过与激活的I-AS基因协同作用进行评估。
Variation in the nucleotide sequence of the HPV 16 E7 gene in preinvasive cervical intraepitherial neoplasia (CIN) and invasive cervical carcinoma specimens was analyzed. Direct DNA sequencing of PCR-amplified products with primers different fram those used for PCR with 5'-end labeling generated distinct sequence ladders with a low background, even in specimens containing relatively low copy numbers of HPV. Of 14 cervical neoplasias, II cases showed sequence diversity from prototype HPV16, and a total of 22 nucleotide exchanges were detected. Nine of these led to single amino acid exchanges: [Thrs] to [Lys(5)] in one case and [Asn(29)] to [Ser(29)] in eight cases. The [Ser(29)] E7 was distributed uniformly among invasive carcinomas and precancerous legions, and was also found in a normal cervix. The [Lys(5)] E7 and [Ser(29)] E7 had transforming potential similar to the prototype E7 assessed by cooperation with the activated I as gene in rat embryo fibroblasts.