The nucleolar phosphoprotein B23 targets Newcastle disease virus matrix protein to the nucleoli and facilitates viral replication
The nucleolar phosphoprotein B23 targets Newcastle disease virus matrix protein to the nucleoli and facilitates viral replication
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核仁磷蛋白 B23 将新城疫病毒基质蛋白靶向核仁并促进病毒复制。
DOI：
10.1016/j.virol.2014.01.011
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发表时间：
2014-03-01
期刊：
影响因子：
3.7
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
--
作者：
Duan, Zhiqiang;Chen, Jian;Liu, Xiufan
据报道，细胞核仁蛋白通过与病毒蛋白相互作用促进一些人和动物病毒的复制周期。在本研究中，一个核仁磷蛋白B23被鉴定为与纽卡斯尔病毒（NDV）基质（M）蛋白相互作用。结果表明，NDV M蛋白在感染早期通过与B23结合而聚集在核仁中，但在感染后期，B23从核仁重新分布到核质中。利用缺失突变体进行的体外结合研究表明，结合需要M的氨基酸30-60和B23的氨基酸188-245。此外，通过siRNA敲低B23或过表达B23或M结合B23衍生多肽显著降低细胞病变效应并抑制NDV复制。总的来说，我们表明，B23促进NDV复制的靶向M的核仁，第一次证明了核仁蛋白B23在副粘病毒复制过程中的直接作用。(C)2014爱思唯尔公司All rights reserved.
The cellular nucleolar proteins are reported to facilitate the replication cycles of some human and animal viruses by interaction with viral proteins. In this study, a nucleolar phosphoprotein B23 was identified to interact with Newcastle disease virus (NDV) matrix (M) protein. We found that NDV M protein accumulated in the nucleolus by binding B23 early in infection, but resulted in the redistribution of B23 from the nucleoli to the nucleoplasm later in infection. In vitro binding studies utilizing deletion mutants indicated that amino acids 30-60 of M and amino acids 188-245 of B23 were required for binding. Furthermore, knockdown of B23 by siRNA or overexpression of B23 or M-binding B23-derived polypeptides remarkably reduced cytopathic effect and inhibited NDV replication. Collectively, we show that B23 facilitates NDV replication by targeting M to the nucleolus, demonstrating for the first time a direct role for nucleolar protein B23 in a paramyxovirus replication process. (C) 2014 Elsevier Inc. All rights reserved.