TeraVR empowers precise reconstruction of complete 3-D neuronal morphology in the whole brain
TeraVR empowers precise reconstruction of complete 3-D neuronal morphology in the whole brain
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TeraVR 能够精确重建全脑完整的 3D 神经元形态
DOI：
10.1038/s41467-019-11443-y
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发表时间：
2019-08-02
影响因子：
16.6
通讯作者：
Peng, Hanchuan
中科院分区：
文献类型：
--
作者：
Wang, Yimin;Li, Qi;Peng, Hanchuan
神经元形态被认为是细胞类型的一个关键决定因素，但哺乳动物神经元的完整的三维（3-D）形态的定量分析仍然是艰巨的，当神经元具有复杂的树枝状和长的投影。神经元形态的全脑重建更具挑战性，因为它涉及处理数十个太体素的成像数据。验证这样的重建是非常费力的。我们开发了开源虚拟现实注释系统TeraVR来应对这些挑战。TeraVR集成了太体像素级图像的沉浸式和协作式3D可视化、交互和分层流。使用TeraVR，我们已经在整个小鼠大脑中产生了精确的长投射神经元的3D完整形态，并开发了一个高度精确的神经元重建的协作工作流程。
Neuron morphology is recognized as a key determinant of cell type, yet the quantitative profiling of a mammalian neuron's complete three-dimensional (3-D) morphology remains arduous when the neuron has complex arborization and long projection. Whole-brain reconstruction of neuron morphology is even more challenging as it involves processing tens of teravoxels of imaging data. Validating such reconstructions is extremely laborious. We develop TeraVR, an open-source virtual reality annotation system, to address these challenges. TeraVR integrates immersive and collaborative 3-D visualization, interaction, and hierarchical streaming of teravoxel-scale images. Using TeraVR, we have produced precise 3-D full morphology of long-projecting neurons in whole mouse brains and developed a collaborative workflow for highly accurate neuronal reconstruction.