Electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor
Electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor
复制标题
DOI：
10.1016/j.bioelechem.2009.09.013
复制
发表时间：
2010-08-01
影响因子：
5
通讯作者：
Ramanaviciene, A.
中科院分区：
文献类型：
--
作者：
Ramanavicius, A.;Finkelsteinas, A.;Ramanaviciene, A.
聚吡咯（Ppy）是一种基于电化学阻抗谱（EIS）测量的无标记电化学免疫传感器的基质。本文提出的免疫传感系统模型是基于电化学合成的聚吡咯（Ppy/gp 51）包埋牛白血病病毒（BLV）蛋白（gp 51）。该Ppy/gp 51层与抗gp 51的抗体（抗gp 51-Ab）相互作用，所述抗体以显著浓度存在于BLV感染牛的血清中。在这种相互作用之后，形成蛋白质复合物（Ppy/gp 51/anti-gp 51-Ab）。辣根过氧化物酶（HRP）标记的抗gp 51-Ab二抗（Ab*）与Ppy/gp 51/anti-gp 51-Ab相互作用形成大蛋白复合物（Ppy/gp 51/anti-gp 51-Ab/Ab*）。对用本文所述的不同Ppy层修饰的电极进行了EIS研究，并采用最佳等效电路来评估EIS谱，这是本研究的主要成果。(C)2009 Elsevier B. V.保留所有权利。
Polypyrrole (Ppy) has been shown as a matrix for label-free electrochemical immunosensor based on electrochemical impedance spectroscopy (EIS) measurements. The immunosensing system model presented here was based on bovine leukemia virus (BLV) protein (gp51) entrapped within electrochemically-synthesized polypyrrole (Ppy/gp51). This Ppy/gp51 layer interacted with antibodies against gp51 (anti-gp51-Ab) that are present in significant concentration in the blood serum of BLV infected cattle. After this interaction protein complex (Ppy/gp51/anti-gp51-Ab) was formed. The horseradish peroxidase (HRP) labeled secondary antibodies (Ab*) against anti-gp51-Ab were applied as agents interacting with Ppy/gp51/anti-gp51-Ab and forming the large protein complex (Ppy/gp51/anti-gp51-Ab/Ab*). The EIS study was performed for electrodes modified with different Ppy layers described here and an optimal equivalent circuit was adopted for evaluation of EIS spectra, it was a major outcome of this study. (C) 2009 Elsevier B.V. All rights reserved.