Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors
Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors
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DOI：
10.1016/j.neuron.2005.08.028
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发表时间：
2005-10-06
期刊：
影响因子：
16.2
通讯作者：
Fishell, G
中科院分区：
文献类型：
--
作者：
Machold, R;Fishell, G
我们已经利用了体内诱导的遗传-命运-映射策略，永久标记的Math 1阳性细胞和它们的后代，出现在小脑原基的菱形唇胚胎发育过程中的队列。在E12.5之前的阶段，除了小脑深部核，我们发现Math 1细胞迁移出小脑原基进入喙状后脑，以填充特定的核，包括中脑脑桥被盖系统的胆碱能神经元。此外，对Math 1无效胚胎的分析表明，该基因是形成其中一些细胞核所必需的。在E12.5左右，颗粒细胞前体开始被标记：首先，产生颗粒细胞的颗粒细胞主要分布在成人小脑的前叶，然后，那些颗粒细胞分布在更靠近尾叶的区域，直到E17完成所有颗粒细胞前体的标记。因此，我们证明，小脑菱形唇引起多种细胞类型的菱形1。
We have utilized an in vivo-inducible genetic-fate-mapping strategy to permanently label cohorts of Math1-positive cells and their progeny that arise in the rhombic lip of the cerebellar primordium during embryogenesis. At stages prior to E12.5, with the exception of the deep cerebellar nuclei, we find that Math1 cells migrate out of the cerebellar primordium into the rostral hindbrain to populate specific nuclei that include cholinergic neurons of the mesopontine tegmental system. Moreover, analysis of Math1-null embryos shows that this gene is required for the formation of some of these nuclei. Around E12.5, granule cell precursors begin to be labeled: first, ones that give rise to granule cells that predominantly populate the anterior lobes of the adult cerebellum and later, those that populate progressing more caudally lobes until labeling of all granule cell precursors is complete by E17. Thus, we demonstrate that the cerebellar rhombic lip gives rise to multiple cell types within rhombomere 1.