Acid-adapted Listeria monocytogenes displays enhanced tolerance against the lantibiotics nisin and lacticin 3147.
Acid-adapted Listeria monocytogenes displays enhanced tolerance against the lantibiotics nisin and lacticin 3147.
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酸适应的单核细胞增生李斯特氏菌对羊毛硫抗生素乳链菌素和乳酸菌素 3147 表现出增强的耐受性。
DOI：
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发表时间：
1999
影响因子：
2
通讯作者：
Colin Hill
中科院分区：
文献类型：
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作者：
W. Schaik;C. Gahan;Colin Hill
对数相单核增生李斯特菌细胞在pH为5.5的酸适应条件下对各种环境胁迫具有耐受性。我们证明，适应的细胞也表现出对nisin的耐受性增加，并在较小程度上对乳酸菌素3147的耐受性增加。在nisin浓度为100和200 IU/ml时，酸适应细胞的存活率约为未适应细胞的10倍。然而，酸适应对单核增生乳杆菌对乳酸菌素3147的耐受性只有适度的影响，这一现象可能反映了这种细菌素的独特作用模式。细菌膜脂肪酸组成分析表明，酸适应细胞中直链脂肪酸C14:0和C16:0显著增加，而C18:0水平下降。结果表明，在轻度酸性条件下诱导的应激机制对细菌素的抗菌作用提供了保护。适应酸的单核细胞增生乳杆菌的这种抗性增加可能导致这种病原体在使用乳酸链球菌素或其他细菌素作为防腐剂的食品中的存活率增加。
Log-phase Listeria monocytogenes cells become tolerant to a variety of environmental stresses following acid adaptation at pH 5.5. We demonstrated that adapted cells also exhibit increased tolerance to nisin and, to a lesser extent, lacticin 3147. At nisin concentrations of 100 and 200 IU/ml the survival of acid-adapted cells was approximately 10-fold greater than nonadapted cells. However, acid adaptation had only a moderate effect on the tolerance of L. monocytogenes to lacticin 3147, a phenomenon that possibly reflects the distinct mode of action of this bacteriocin. Analysis of the fatty acid composition of the bacterial membrane indicated that straight-chain fatty acids C14:0 and C16:0 were significantly increased in acid-adapted cells while levels of C18:0 decreased. The results indicate that stress mechanisms that are induced in mildly acidic conditions provide protection against the antimicrobial action of bacteriocins. This increased resistance of acid-adapted L. monocytogenes could cause increased survival of this pathogen in food products in which nisin or other bacteriocins are used as preservatives.