Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis
Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis
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DOI：
10.1016/j.clinbiochem.2015.10.006
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Barisic, Karmela
中科院分区：
文献类型：
--
作者：
Galovic, Ruzica;Flegar-Mestric, Zlata;Barisic, Karmela
目的：热休克蛋白（Hsps）由包括血管在内的所有细胞产生，以确保应激保护。受损细胞在局部环境和体循环中释放热休克蛋白。本研究的目的是探讨血清Hsp60和Hsp70以及各自的抗Hsp60和抗Hsp70抗体在晚期动脉粥样硬化导致脑血管高度狭窄的受试者中的作用和预后价值。设计和方法：采用颈动脉超声多普勒检查来区分对照组和脑血管粥样硬化组。选取28名无颈动脉梗阻的受试者作为对照。采用数字减影血管造影对50例脑血管血流阻塞患者的脑动脉狭窄程度进行评价。同时用ELISA试剂盒检测血清Hsp60、Hsp70、抗Hsp60和抗Hsp70的浓度。结果：动脉粥样硬化组抗hsp60明显高于对照组（23.62 ng/L vs 15.28 ng/L，以中位数表示）（P = 0.003）。动脉粥样硬化组循环Hsp70低于对照组（P = 0.048），中位值分别为0.00 μ g/L和0.22 μ g/L。Hsp60和抗hsp70的浓度在对照组和动脉粥样硬化组之间无显著差异。结论：较高的循环抗hsp60与晚期脑血管粥样硬化有关，这是动脉粥样硬化炎症和破裂的自身免疫部分的结果。在对照组中观察到的较高水平的Hsp70可能对动脉粥样硬化变化的发展具有保护作用。(C) 2015加拿大临床化学学会。Elsevier Inc.出版。版权所有。
Objectives: Heat shock proteins (Hsps) are produced by all cells, including vascular, to ensure stress protection. Damaged cells release Hsps in their local environment and systemic circulation. The aim of this study was to investigate the involvement and prognostic utility of serum Hsp60 and Hsp70, and the respective antibodies anti-Hsp60 and anti-Hsp70 in subjects with advanced atherosclerosis resulting in high degree of cerebrovascular stenosis.Design and methods: Ultrasound Doppler examination of carotid arteries was used to discriminate between control and cerebrovascular atherosclerosis subjects. Twenty eight subjects without carotid obstruction were selected as controls. Fifty patients with obstruction of cerebrovascular blood flow were evaluated for the degree of stenosis of cerebral arteries by digital subtraction angiography. In parallel, serum concentrations of Hsp60, Hsp70, anti-Hsp60 and anti-Hsp70 were measured by ELISA kits.Results: Anti-Hsp60 was significantly higher (P = 0.003) in the atherosclerosis group than in the control group (23.62 ng/L vs. 15.28 ng/L, respectively, expressed as median). Circulating Hsp70 was lower in the atherosclerosis than in the control group (P = 0.048), with respective median values of 0.00 mu g/L vs. 0.22 mu g/L. Concentrations of Hsp60 and anti-Hsp70 did not differ significantly between the control and atherosclerosis group.Conclusions: Higher circulating anti-Hsp60 is associated with advanced cerebrovascular atherosclerosis as a consequence of the autoimmunity part of the inflammation and bursting of atherosclerosis. Higher levels of Hsp70 observed in the control group could be protective in the development of atherosclerotic changes. (C) 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.