DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly
DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly
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基于 DNAzyme 的逻辑门介导的 DNA 自组装
DOI：
10.1021/acs.nanolett.5b04608
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发表时间：
2016
期刊：
影响因子：
10.8
通讯作者：
Yan Hao
中科院分区：
文献类型：
--
作者：
Zhang Cheng;Yang Jing;Jiang Shuoxing;Liu Yan;Yan Hao
使用合理设计的逻辑门控制DNA自组装过程是基于DNA的纳米技术和编程的主要目标。这样的控制可以促进响应于各种分子触发器或输入的复杂纳米粒子的分级工程。在这里，我们展示了使用一系列的基于DNA酶的逻辑门来控制DNA瓦片自组装到规定的DNA折纸框架。诸如“是”、”或“、”与”和“逻辑开关”的逻辑系统基于DNA酶介导的瓦片识别与DNA折纸框架来实现。DNAzyme被设计成扮演两个角色：（1）作为中间信使以激发下游反应，以及（2）作为最终触发以报告荧光信号，使得能够在DNA折纸框架瓦片组装和荧光信号传导之间进行信息中继。本研究的结果证明了DNA酶介导的分级自组装的可行性，并为产生动态和响应性自组装系统提供了新的工具。
Controlling DNA self-assembly processes using rationally designed logic gates is a major goal of DNA-based nanotechnology and programming. Such controls could facilitate the hierarchical engineering of complex nanopatterns responding to various molecular triggers or inputs. Here, we demonstrate the use of a series of DNAzyme-based logic gates to control DNA tile self-assembly onto a prescribed DNA origami frame. Logic systems such as “YES,” “OR,” “AND,” and “logic switch” are implemented based on DNAzyme-mediated tile recognition with the DNA origami frame. DNAzyme is designed to play two roles: (1) as an intermediate messenger to motivate downstream reactions and (2) as a final trigger to report fluorescent signals, enabling information relay between the DNA origami-framed tile assembly and fluorescent signaling. The results of this study demonstrate the plausibility of DNAzyme-mediated hierarchical self-assembly and provide new tools for generating dynamic and responsive self-assembly systems.