Holographic Microscopy
Holographic Microscopy
复制标题
全息显微镜
作者：
R. F. vanLigten;H. Osterberg
Gabor 2和Parrent等人3认识到在可见光谱中的有限类别的物体上使用全息术1，但是由于零阶光的存在，在重建图像的对比度中出现了问题，因为没有使用物体和参考光束的角度干涉。利斯和乌帕特尼克斯[4]以及罗曼[5]已经描述了一种技术，这种技术使得在更一般的和非微观的物体上进行全息照相成为可能。利斯和Upatnieks6使用相同的技术在小物体上进行全息摄影，而不使用透镜。在我们对更一般的物体进行的实验中，当没有透镜进行全息照相时，最佳的可探测性下限约为12μ。然而，获得约5μ的分辨率的实验困难是非常严重的。我们用显微镜（物镜透镜）记录具有一般特征的全息显微图像。虽然没有使用“无透镜”图像的吸引人的特征，但保留了记录物体中的实质性深度以及相位信息的能力。
THE use of holography1 on a restricted class of objects in the visible spectrum was recognized by Gabor2 and Parrent et al.3, but a problem occurred in the contrast of the reconstructed image owing to the presence of zero-order light, as no angular interference of the object and reference beams was used. Leith and Upatnieks4 as well as Lohman5 have described a technique which makes possible holography on more general and non-microscopic objects. This same technique was used to perform holography without the use of a lens on small objects by Leith and Upatnieks6. In experiments which we have carried out on more general objects, the optimum lower level of detectability was about 12µ when holography was performed without a lens. However, the experimental difficulties of obtaining a resolution of about 5µ are very serious. We have used a microscope (objective lens) to record holographically microscopic images of a general character. Although the attractive feature of ‘lens-less’ imagery was not used, the ability to record a substantial depth, as well as phase information in the object, has been preserved.