Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice
Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice
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DOI：
10.1016/j.acthis.2010.06.006
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发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Yang, Tian
中科院分区：
文献类型：
--
作者：
Xing, YiZhan;Xu, Wei;Yang, Tian
以往的研究表明，Wnt信号通路在毛囊的生长发育中起着重要作用。Wnt5a是一种非规范的Wnt基因，它抑制Wnt/β-catenin信号通路，已被广泛接受。已有报道报道其在胚胎和出生后毛囊中的表达，但目前尚不清楚其在毛囊生长中的确切作用。在本研究中，我们研究了Wnt5a蛋白在小鼠背部皮肤小球和胡须毛囊中的免疫定位。我们发现，在生长期，毛乳头细胞Wnt5a蛋白的染色水平最高，而在退化期和休止期，Wnt5a蛋白的染色水平较低。在生长期，除内根鞘、外根鞘和毛乳头外，Wnt5a蛋白主要分布在基质和皮层前细胞中。随着毛发周期的进展，Wnt5a的免疫染色在退化期逐渐减弱，在退化期定位于隆起和次生毛胚中。这一WNT5a免疫染色图谱在背部皮肤小球和胡须毛囊之间是一致的。此外，在使用胡须毛囊器官培养的体外研究中，我们发现腺病毒Wnt5a显著抑制了毛干的生长。我们的研究结果表明，Wnt5a是毛发周期中的一个动态因子，对毛干生长的调节很重要。(C)2010年爱思唯尔股份有限公司。版权所有。
Previous studies have shown that the Wnt signaling pathway plays an important role in the growth and development of hair follicles. It has been generally accepted that Wnt5a, a non-canonical Wnt gene, inhibits the Wnt/beta-catenin signaling pathway. Several reports have addressed its mRNA expression in embryonic and postnatal hair follicles, but its exact role in the growth of hair follicles is currently unknown. In this study, we investigated the immunolocalization of Wnt5a protein in pelages of the dorsal skin and whisker follicles of mice. We found that in the anagen phase, dermal papilla cells showed the highest staining levels of Wnt5a protein, while in the catagen and the telogen phases the staining levels were lower. During the growth stage, Wnt5a protein was prominently located in the matrix and precortex cells in addition to the inner root sheath, outer root sheath and the dermal papilla. As the hair cycle progresses, the immunostaining of Wnt5a was gradually decreased in the catagen phase and was located in the bulge and secondary hair germ in the telogen phase. This Wnt5a immunostaining profile was consistent between dorsal skin pelages and whisker follicles. Furthermore, in an in vitro study using whisker follicle organ culture, we demonstrated that the growth of the hair shaft was significantly inhibited by adenovirus Wnt5a. Our findings suggest that Wnt5a is a dynamic factor in the hair cycle and it is important for the regulation of hair shaft growth. (C) 2010 Elsevier GmbH. All rights reserved.