Factors required for the uridylylation of the foot-and-mouth disease virus 3B1, 3B2, and 3B3 peptides by the RNA-dependent RNA polymerase (3Dpol) in vitro
Factors required for the uridylylation of the foot-and-mouth disease virus 3B1, 3B2, and 3B3 peptides by the RNA-dependent RNA polymerase (3Dpol) in vitro
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DOI：
10.1128/jvi.79.12.7698-7706.2005
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Belsham, GJ
中科院分区：
文献类型：
--
作者：
Nayak, A;Goodfellow, IG;Belsham, GJ
小核糖核酸基因组RNA的5'端与病毒编码的肽313 （VTg）共价连接。口蹄疫病毒（FMDV）在编码和使用3种不同形式的这种肽方面是独一无二的。这些肽都是病毒编码的RNA聚合酶3D（pol）合成RNA的引物。为了作为正链RNA合成的引物，3B肽必须被水解以形成VPgpU（pU）。对于某些小核糖核酸病毒，已经证明这种反应是通过3D（pol）在3CD前体和称为顺式作用复制元件（cre）的内部RNA序列存在下实现的。FMDV先前已被鉴定为位于5'非翻译区，而所有其他小核糖核酸核酸结构都位于病毒编码区。现在已经确定了每个FMDV 313肽的体外尿苷化要求，并分析了FMDV ere（也称为3b -尿苷化位点，或bus）在该反应中的作用。聚(A)尾部不作为FMDV 3B尿苷化的重要模板。
The 5' terminus of picornavirus genomic RNA is covalently linked to the virus-encoded peptide 313 (VTg). Foot-and-mouth disease virus (FMDV) is unique in encoding and using 3 distinct forms of this peptide. These peptides each act as primers for RNA synthesis by the virus-encoded RNA polymerase 3D(pol). To act as the primer for positive-strand RNA synthesis, the 3B peptides have to be uridylylated to form VPgpU(pU). For certain picornaviruses, it has been shown that this reaction is achieved by the 3D(pol) in the presence of the 3CD precursor plus an internal RNA sequence termed a cis-acting replication element (cre). The FMDV ere has been identified previously to be within the 5' untranslated region, whereas all other picornavirus cre structures are within the viral coding region. The requirements for the in vitro uridylylation of each of the FMDV 313 peptides has now been determined, and the role of the FMDV ere (also known as the 3B-uridylylation site, or bus) in this reaction has been analyzed. The poly(A) tail does not act as a significant template for FMDV 3B uridylylation.