GENETIC-ANALYSIS OF VIRULENCE IN THE RICE BLAST FUNGUS MAGNAPORTHE-GRISEA
GENETIC-ANALYSIS OF VIRULENCE IN THE RICE BLAST FUNGUS MAGNAPORTHE-GRISEA
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DOI：
10.1094/phyto-78-1227
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发表时间：
1988-09-01
期刊：
影响因子：
3.2
通讯作者：
NOTTEGHEM, JL
中科院分区：
文献类型：
--
作者：
LEUNG, H;BORROMEO, ES;NOTTEGHEM, JL
利用稻瘟病菌不同分离株间杂交，研究了稻瘟病菌对水稻致病力的遗传控制。两性水稻分离物Guy 11和实验室菌株2539之间的杂交产生了超过50%的活子囊孢子。随机孢子和四分体分析表明，两个位点，Pos 1（对Orva sativa的致病性）和Pos 2，参与调节对水稻51583和沙条草的致病性。浅黄色突变对毒力具有上位性。田间稻株间杂交的育性和子囊孢子活力较低。两个水稻分离物的杂交。Guy 11和CH 104-3的后代中，只有10%的子囊孢子对51583和沙条草有致病力。从这个交叉，我们确定了两个不同的位点，Pos 3和Pos 4，控制水稻株系K59和Kinandang巴东，分别毒力。从F2和F1代恢复的后代保持了育性和毒力。联合分离分析表明，过量的亲本类型的毒力。这可能反映了毒力基因的遗传连锁或其他遗传因素对毒力的上位性分离。
Crosses between rice isolates of Magnaporthe grisea were made to determine the genetic control of virulence to rice. A cross between a hermaphroditic rice isolate, Guy 11, and a laboratory strain 2539, yielded more than 50% viable ascospores. Random spore and tetrad analysis showed that two loci, Pos1 (pathogenicity on Orva sativa) and Pos2, were involved in conditioning virulence to rice lines 51583 and Sha-tiao-tsao. A buff mutation was epistatic to virulence. Fertility and ascospore viability were low in crosses between field rice isolates. In a cross between two rice isolates. Guy 11 and CH 104-3, only 10% of the ascospores was biable, but all progeny were virulent to 51583 and Sha-tiao-tsao. From this cross, we identified two different loci, Pos3 and Pos4, controlling virulence on rice lines K59 and Kinandang Patong, respectively. Fertility and virulence were maintained in progeny recovered from F2 and F1 generations. Joint segregation analysis showed an excess of parental types with respect to virulence. This might reflect genetic linkage of virulence genes or segregation of other genetic factors epistatic to virulence.